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1、VIROLOGY157,47-57(1987)SequenceAnalysisoftheBovineCoronavirusNucleocapsidandMatrixProteinGenesWILLIAMIAPPS,BRENDAG.HOGUE,ANDDAVIDA.BRIANDepartmentofMicrobiology,TheUniversityofTennessee,Knoxville,Tennessee37996-0845ReceivedJuly17,1986;acceptedOctober22,1986The3en
2、doftheZO-kbgenomeoftheMebusstrainofbovineentericcoronavirus(BCV)wascopiedintocDNAandclonedintothe/JstlsiteofthepUC9vector.Fourclonesfromthe3endofthegenomeweresequencedeithercompletelyorinparttodeterminethesequenceofthefirst2451bases.Withinthissequencewereidentifi
3、ed,inorder,a3-noncodingregionof291bases,thegenefora449-aminoacidnucleocapsidprotein(N)havingamolecularweightof49,379,andthegenefora230-aminoacidmatrixprotein(M)havingamolecularweightof26,376.AthirdlargeopenreadingframeiscontainedentirelywithintheNgenesequencebuti
4、spositionedinadifferentreadingframe;itpotentiallyencodesapolypeptideof207aminoacidshavingamolecularweightof23,057.AhigherdegreeofaminoacidsequencehomologywasfoundbetweentheMproteinsofBCVandMHV(87%)thanbetweentheNproteins(70%).FortheMproteinsofBCVandMHV,notabledif
5、ferenceswerefoundattheaminoterminus,themostprobablesiteof0-glycosylation,wherethesequenceisN-Met-Ser-Ser-Val-Thr-ThrforBCVandN-Met-Ser-Ser-Thr-ThrforMHV.BCVapparentlyusestwoofitssixpotentialC-glycosylationsites.o1987Academicpress,inc.INTRODUCTIONgeneonthegenomean
6、dtheresultingdivergencefromtheMHVgenomestructure.Thebovineentericcoronavirus(BCV)isonecauseInthispaperwedescribeexperimentsthatbegintoofsevereenteritisincalvesandmayberesponsibleexaminetheBCVgenomebycDNAcloningandDNAforasmuchasone-quarterofalldeathsduetothisseque
7、ncing.Withinthe32451-basesequencewefinddisease(House,1978).VaccinesproducedfromcellagenemapthatparallelsthatforMHV.Wereporttheculture-attenuatedstrainsofvirushavefailedtobeprimarystructurefortheNandMgenesandtheirde-completelyprotective.Beforeattemptingtodevelopdu
8、cedaminoacidsequences.StructuralcomparisonsvaccinesbyrecombinantDNAthatmayhaveimprovedwithothercoronavirusNandMsequencesaremadeusefulness,itisimperativethatthe
