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1、VeterinaryMicrobiology,22(1990)187-201187ElsevierSciencePublishersB.V.,Amsterdam--PrintedinTheNetherlandsALongitudinalStudyofBovineCoronavirusEntericandRespiratoryInfectionsinDairyCalvesinTwoHerdsinOhio*R.A.HECKERT,L.J.SAIF,K.H.HOBLETandA.G.AGNESFoodAnimalHealthResearchProgram,OhioAgr
2、iculturalResearchandDevelopmentCenter,TheOhioStateUniversity,WoosterOH,44691(U.S.A.)(Accepted24October1989)ABSTRACTHeckert,R.A.,Saif,L.J.,Hoblet,K.H.andAgnes,A.G.,1990.AlongitudinalstudyofbovinecoronavirusentericandrespiratoryinfectionsindairycalvesintwoherdsinOhio.Vet.Micro-biol.,22:
3、187-201.Thisprospectivelongitudinalstudyexaminedtheepidemiologyanddiseasesyndromeassoci-atedwithbovinecoronavirus(BCV)infectionsinacohortof8conventionalcalvesfrom0to120daysofage,intwodairyherdsinOhio.TheperiodsofrespiratorysheddingofBCVweredeter-minedbydirectimmunofluorescent(DIF)stai
4、ningofnasalepithelialcellsandELISAofnasalswabsupernatantfluids.TheperiodsoffecalsheddingofBCVweredeterminedbyELISAandimmunoelectronmicroscopy(IEM).Theisotype-specificantibodytiterstoBCVinserum(atselectedintervalsbetween0and120daysofage)andthepost-suckling(24to48hafterbirth)totalimmuno
5、globulinlevelswereexaminedbyELISAandzincsulfateturbiditytests,respectively.Ofthe8calvesstudied,4hadevidenceofBCVrespiratory(byDIForELISA)orentericinfections(byIEMorELISA)inassociationwithdiarrheaorrhinitis,eventhough7of8calvesshowedincreasesinoneormoreserumantibodyisotypestoBCVand6of8
6、calvesshowedBCVrespiratoryorentericantigensheddingbyELISA.Serologicalantibodytiterincreasesoccurredin3calvesbefore30daysofageandin4calvesafter30daysofage;twoofthelattercalveshadasecondriseinserumantibodytiterstoBCVaftertheinitialrise.Aserologicalantibodytiterincreasewasnotobservedinon
7、ecalf.ThissuggeststhatBCVinfectionsmaybeverycommoninaclosedherdandmayoccurinoldercalves,althoughmanymaybesubclinicalandsomemayberecurrent.TherewerenostatisticallysignificantcorrelationsbetweentotalserumimmunoglobulinlevelsorBCVantibodyisotypetitersinserum(24-48hafterbirth)andclinicald
8、iseas
