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1、SHANDONGＵＮＩＶＥＲＳＩＴＹ?。希啤。裕牛茫龋危希蹋希牵佼厴I(yè)論文玉米芯誘導(dǎo)里氏木霉合成纖維素酶學(xué)院：專業(yè)班級(jí)：學(xué)生姓名：學(xué)號(hào)：指導(dǎo)教師：201年月32目錄摘要纖維素類物質(zhì)是自然界中最具潛力的一種可再生資源，其有效利用是目前人們關(guān)注的熱點(diǎn)。作為降解纖維素的多組分酶系，纖維素酶降解纖維素特異性高，反應(yīng)條件溫和，環(huán)境污染小。但由于其組分復(fù)雜，合成代謝網(wǎng)絡(luò)研究尚不清楚。此外，真菌纖維素酶多數(shù)是誘導(dǎo)酶，其合成須在誘導(dǎo)物存在下才能進(jìn)行，通過添加誘導(dǎo)物及對(duì)纖維素酶合成途徑進(jìn)行調(diào)控是提高纖維素酶活性的有效方法。因此，研究以天然纖維素物質(zhì)為底物的纖維素酶合成過程機(jī)理，充分挖掘現(xiàn)有生產(chǎn)菌株產(chǎn)纖維素酶的潛
2、力，是解決纖維素酶誘導(dǎo)合成問題的關(guān)鍵。本文對(duì)天然纖維素物質(zhì)玉米芯誘導(dǎo)里氏木霉產(chǎn)纖維素酶的過程進(jìn)行了分析，通過測(cè)定玉米芯酶解液中糖的組分，并以所測(cè)定到的組分為誘導(dǎo)物進(jìn)行誘導(dǎo)產(chǎn)酶，發(fā)現(xiàn)除纖維素成分外，半纖維素水解產(chǎn)物半乳糖對(duì)纖維素酶的合成有誘導(dǎo)作用。通過優(yōu)化半乳糖的誘導(dǎo)濃度，發(fā)現(xiàn)5g/L的半乳糖對(duì)纖維素酶的誘導(dǎo)最好。關(guān)鍵詞：里氏木霉、纖維素酶、玉米芯、誘導(dǎo)32目錄AbstractCelluloseisanaturalsubstanceinthemostpotentialasarenewableresource,anditsefficientuseiscurrentlythefocusofatte
3、ntion.Asamulti-componentenzymesdegradecellulose,cellulosedegradationofcellulosehighspecificity,mildreactionconditions,environmentalpollution.However,duetothecomplexityofitscomponents,anabolicNetworkResearchunclear.Inaddition,mostofthefungalcellulaseenzymeinduction,inductionofthesynthesismustbecarri
4、edoutunderthepresence,andbyaddingtheinducerofcellulasesynthesispathwaytoimprovethecellulaseactivityregulatingeffectiveway.Therefore,thestudyofnaturalcellulosicmaterialsassubstratescellulasesynthesismechanismtofullyexploittheexistingstrainsproducingcellulaseproductionpotentialistosolvetheproblemofli
5、quidfermentationofcellulasekey.Inthispaper,naturalcellulosesubstancescorncobinducedTrichodermareeseicellulaseproductionprocesswasanalyzedbymeasuringthesugarincorncobhydrolyzatecomponents,andthegrouptowhichthemeasuredinducerforinductionintoproductionenzymes,foundthatinadditioncelluloseingredients,he
6、micellulosehydrolyzategalactoseoncellulasesynthesisinduced.Byoptimizingtheconcentrationofgalactoseinduction,found5g/Lofgalactoseinductionofcellulasebest.Keywords:Trichodermareesei,cellulase,corncob,inducing32目錄目錄摘要IABSTRACTII目錄III第一章緒論研究進(jìn)展，立項(xiàng)依據(jù)11.1里氏木霉簡介11.2木質(zhì)纖維素原料的組成11.3纖維素酶研究進(jìn)展21.3.1纖維素酶的性質(zhì)和種類31.
7、3.2纖維素酶的作用機(jī)理41.3.3纖維素酶的來源41.3.4纖維素酶的應(yīng)用51.4里氏木霉產(chǎn)纖維素酶的誘導(dǎo)物61.4.1纖維素61.4.2槐糖71.4.3乳糖71.4.4纖維二糖71.5立項(xiàng)依據(jù)71.6本文研究問題8第二章材料方法102.1菌種102.2主要實(shí)驗(yàn)儀器102.3實(shí)驗(yàn)藥品112.4實(shí)驗(yàn)試劑122.4.1檸檬酸緩沖液122.4.2DNS反應(yīng)液122.4.3微量元素122.5實(shí)驗(yàn)培養(yǎng)基122.5.1
