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1、VeterinaryMicrobiology,1(1976)65--6965?ElsevierScientificPublishingCompany,Amsterdam--PrintedinTheNetherlandsREPLICATIONOFABOVINECORONAVIRUSINORGANCULTURESOFFOETALTRACHEAE.J.STOTT,L.H.THOMAS,J.C.BRIDGERandN.J.JEBBETTAgriculturalResearchCouncil,Institute
2、forResearchonAnimalDiseases,Compton,NearNewbury,Berkshire(GreatBritain)(Received26July1976)ABSTRACTStott,E.J.,Thomas,L.H.,Bridger,J.C.andJebbett,N.J.,1976.Replicationofabovinecoronavirusinorganculturesoffoetaltrachea.Vet.Microbiol.,1:65--69.Astrainofbov
3、inecoronavirus(BC)adaptedtotissueculture,wasinoculatedintoor-ganculturesofbovinefoetaltrachea.Haemagglutinininthefluidfrominfectedorganculturesreachedtitresof32andcharacteristiccoronavirusparticleswereobservedelec-tronmicroscopically.BCvirusantigenwasde
4、tectedinfrozensectionsoftheorgancul-turesbystainingwithfluorescentantibody.ThesedatawereevidencethatBCvirusre-plicatedinorganculturesofrespiratorytissue.Theuseofthistechniqueforprimaryiso-lationofbovinecoronavirusesfromfieldmaterialisdiscussed.INTRODUCT
5、IONMostcoronavirusessofardescribedhavetwosignificantbiologicalcharac-teristics.First,theyhaveamarkedtropismforeithertherespiratoryoren-terictractandsecondly,theyaredifficulttoisolateincellculture.Thebovinecoronavirus(BCvirus)isassociatedwithneonataldiar
6、rhoea(Stairetal.,1972),andthusresemblesthevirusoftransmissiblegastroente-ritis(TGE)ofswineandarecentlyreportedhumanvirus(CaulandClarke,1975).Incontrast,avianinfectiousbronchitisvirus(Cunningham,1966),theratcoronavirus(Parkeretal.,1970)andmostcoronavirus
7、esofman(Brad-burneandTyrrell,1971)areassociatedwithrespiratorydisease.However,recentreports(Underdahletal.,1974;Kemenyetal.,1975)indicatethatTGEvirusinfectionhasarespiratoryphase.Therefore,itispossiblethatBCvirusmightreplicateinrespiratorytissue.Thebovi
8、necoronaviruswasoriginallycharacterisedbyanimalpassageafterpurificationfromfaeces(Stairetal.,1972).Lateritwasgrownincellculturewithdifficulty(Mebusetal.,1973a).Sinceinoculationoforgancul-turesoffoetaltrachea(HoornandTyrrell,1969)
