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1、VirusResearch,5(1986)253-263253ElsevierVRR00270ThepeplomerproteinsequenceoftheM41strainofcoronavirusIBVanditscomparisonwithBeaudettestrainsH.G.M.Niesters,J.A.LenstraI,W.J.M.Spaan,A.J.Zijderveld,N.M.C.Bleumink-Pluym,F.Hong4,G.J.M.vanScharrenburg3,M.C.Horzine
2、kandB.A.M.vanderZeijst*InstituteofVirologyand2SectionBacteriology,VeterinaryFaculty,StateUniversity,P.O.Box80.150,3508TDUtrecht,3Duphar,P.0.Box2,1380AAWeesp,TheNetherIan&and4MolecularBiologyandVirologyLaboratory,TheSalkInstitute,SanDiego,California92138,U.S
3、.A.(Acceptedforpublication21April1986)SummaryTheaminoacidsequenceofthegeneforthepeplomerproteinofthevaccinestrainM41andtheBeaudettelaboratorystrainM42-Salkofavianinfectiousbronchitisvirus(IBV)havebeenderivedfromcDNAsequences.Asfoundwithothercoronaviruses,th
4、epeplomsrproteincarriestheepitopeselicitingneutralizingantibodies.Thegeneencodesaprimarytranslationproductof1162aminoacidswithamolecularweightof128079.Theuseofarecentalgorithmtopredictmembrane-proteininteractionsledtotheunambiguouslocalizationofthesignalpep
5、tideandatransmembraneanchora-helixattheC-terminus.At50positionsaminoaciddifferenceswerefoundbetweenM41andtwoBeaudettestrains(M42-SalkandM42-Houghton).Theyarepartlyclusteredintworegionsoftheprotein.Thesetworegionsarecandidatesforneutralizationepitopesofthepr
6、otein.coronavirusIBV,peplomerprotein,strainM41,M42IntroductionInfectiousbronchitisvirus(IBV)ofchickensistheprototypevirusofthefamilyCoronaviridae,agroupofenvelopedviruseswithasingle-strandedcolinearRNAgenomeofpositivesense,approximately20kbinlength(StemandK
7、ennedy,0168-1702/86/$03.5001986ElsevierSciencePublishersB.V.(BiomedicalDivision)254Fig.1.Genomicorganizationofinfectiousbronchitisvirus.ThesequencerelationshipsbetweenthemRNAsareindicated,aswellasthepositionsoftheclonesusedforthesequencingofthegeneencodingp
8、roteinSoftheM41strain.Thegenesforthestructuralproteinsareindicatedbyboxes.Homologiespresentinthegenomeattheintergenicboundariesareindicatedwithverticalbars.Theleadersequencepresentatthe5endisindicated(
