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1、VeterinaryMicrobiology,26(1991)41-5141ElsevierSciencePublishersB.V.,AmsterdamEnzyme-linkedimmunosorbentassayforthedetectionofcaninecoronavirusanditsantibodyindogsKotaroTuchiya*,TaisukeHorimoto,MasayukiAzetaka,EijiTakahashi**andShin-ichiroKonishi***DepartmentofVeterinaryMicrobiology,FacultyofAgricult
2、ure,UniversityofTokyo,1-I-I,Yayoi,Bunkyo-ku,Tokyo113,Japan(Accepted12March1990)ABSTRACTTuchiya,K.,Horimoto,T.,Azetaka,M.,Takahashi,E.andKonishi,S.,1991.Enzyme-linkedimmu-nosorbentassayforthedetectionofcaninecoronavirusanditsantibodyindogs.Vet.Microbiol.,26:41-51.Twomethodsofenzyme-linkedimmunosorben
3、tassay(ELISA)weredevelopedforthediagnosisofcaninecoronavirus(CCV)infectionindogs.OneELISA,inwhichCCV-infectedCRFKcelllysateisusedasantigen,isforthedetectionandtitrationofantibodyagainstCCV,andtheotherELISAusesthedoubleantibodysandwichmethodforthedetectionofCCVantigen.ThefirstELISAproce-duredemonstra
4、tedantibodyresponsesindogsinoculatedwithCCV,asdidthevirusneutralizationtest;thesecondELISAdetectedspecificCCVantigeninfecesandorganhomogenatesofinoculateddogs.INTRODUCTIONCaninecoronavirus(CCV)hasbeenidentifiedasoneofthecausativeagentsofviralenteritisinmembersoftheCanidae.ThepresenceofCCVinfectionha
5、sbeenreportedinmanycountries(Binnetal.,1975;ForeytandEvermann,1985;SchnaglandHolmes,1978;Appeletal.,1979;Vanden-bergheetal.,1980;Tingpalapongetal.,1982;Yasoshimaetal.,1983).Can-ineparvovirus,caninerotavirus,andastrovirusesmayalsoberesponsibleforcanineviralenteritis(CarmichaelandBinn,1981).CCVinfecti
6、onandother*Presentaddress:NipponInstituteforBiologicalScience,2221-1,Shinmachi,Ome,Tokyo198,Japan.**Towhomcorrespondenceshouldbeaddressed.***Presentaddress:DepartmentofAnimalHealthI,SchoolofVeterinaryMedicine,AzabuUni-versity,1-17-71,Fuchinobe,Sagamihara,Kanagawa229,Japan.0378-1135/91/$03.50?1991--E
7、lsevierSciencePublishersB.V.42K.TUCHIYAETAL.viralentericinfectionsarehighlycontagiousandspreadrapidlythroughdogpopulations.ClinicalsignsofCCVinfectionvaryfrominapparenttothoseofrapidlyfatalgastroenter
