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《冠狀病毒Coronavirusl論文-1994 Antigen detection in human respiratory Coronavirus infections by monoclonal time-resolved fluoroimmunoassay.pdf》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、~ClinicalandDiagnosticClinicalandDiagnosticVirologyVirologyELSEVIER2(1994)165-179AntigendetectioninhumanrespiratoryCoronavirusinfectionsbymonoclonaltime-resolvedfluoroimmunoassayJohnC.Hierholzer"'*,PekkaE.Halonena,b,PatriciaG.Bingham",RichardA.Coombs",YvonneO.Stone""R
2、espiratoryandEntericVirusesBranch,DivisionofViralandRickettsialDiseases,CenterforInfectiousDiseases,CentersforDiseaseControl,1600°CliftonRd.N.E.,Atlanta,GA30333,USA;bDepartmentofVirology,UniversityofTurku,Kiinamyllynkatu13,SF-20520,Turku52,FinlandReceived27May1993;rev
3、ised18November1993;accepted1December1993AbstractBackground:Thediagnosisofrespiratoryinfectionsbydetectingviralantigenshasreceivedconsiderableattentionusingimmunofluorescentassays(IFA)andenzymeimmunoassays(EIA).Time-resolvedfluoroimmunoassay(TR-FIA)hasbeendevelopedfors
4、everalviruses.Objectives:Topreparemonoclonalantibodiestocoronavirusstrains,toincorporatethemintoaTR-FIA,andtesttheassayonclinicalspecimens.Studydesign:MonoclonalantibodieswerepreparedtotheNnucleoproteinofthetwohumanrespiratorycoronaviruses,HCVstrains229EandOC43.Monocl
5、onalstobothviruseswerecompletelytype-specific;theydidnotcross-reactbetweenthemselvesorwithmultiplestrainsofotherrespiratoryviruses.TheseantibodieswereconfiguredintooptimizedEIAandTR-FIAtests.Theall-monoclonaltestswerethencomparedtopolyclonalEIAtestsintermsoftheirabili
6、tytodetectvirusinclinicalspecimens.Results:Theall-monoclonalTR-FIAwasuniformlythemostsensitive,detectingvirusinall13229E-positivespecimenscomparedto69%forthemonoclonalEIAand54%forthepolyclonalEIAtest.Similarresultswereobtainedfor10OC43-positivespecimens:100%inTR-FIA,9
7、0%inmonoclonalEIA,and80%inpolyclonalEIA.For229EinTR-FIA,meanpositive/negative(PfN)ratioswere143for229E-positivehumanembryoniclungfibroblast(HLF)cellculturefluidsand10forpositivenasopharyngealaspiratespecimens;forOC43inTR-FIA,meanP/Nvalueswere964forOC43-positiverhabdom
8、yosarcoma(RD)cellculturefluidsand174forpositiveNPAspecimens.ThesensitivitiesoftheTR-FIAweredeterminedwithpurifiedvirionstobe
