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1、Antibody-DependentEnhancementofFelineInfectiousPeritonitisVirusInfectioninFelineAlveolarMacrophagesandHumanMonocyteCellLineU937bySerumofCatsExperimentallyorNaturallyInfectedwithFelineCoronavirusTsutomuHOHDATSU,MikaYAMADA,RitsukoTOMINAGA,KaoriMAKINO,KoujiKI
2、DAandHiroyukiKOYAMADepartmentofVeterinaryInfectiousDiseases,SchoolofVeterinaryMedicineandAnimalSciences,KitasatoUniversity,Towada,Aomori034,Japan(Received3July1997/Accepted28August1997)ABSTRACT.InfectionofthetypeIIfelineinfectiousperitonitisvirus(FIPV)stra
3、in79–1146toprimaryfelinealveolarmacrophagesandhumanmonocytecelllineU937wasenhancedbytheseraofcatsexperimentallyinfectedwiththe79–1146strain,butnotthoseofcatsinfectedwithKU-2orUCD-1strainoftypeIFIPV.Theexperimentsusingseraofcatswithfelineinfectiousperitonit
4、is(FIP)andofcatsnaturallyinfectedwithfelinecoronavirus(FCoV)revealedthatinfectionoftheFIPV79–1146straintotheU937cellswasenhancedonlybytheseraofcatsinfectedwithtypeIIFIPVorfelineentericcoronavirus.Thesamplespositiveforantibody-dependentenhancement(ADE)activ
5、ityhadhighneutralizingantibodytitersagainsttheFIPV79–1146strainandthesamplesnegativeforADEactivityhadlowneutralizingantibodytiters.ThesefindingssupportthepreviousresultswhereamonoclonalantibodywithneutralizingactivityhadhighADEactivity,suggestingthattherew
6、asacloserelationshipbetweentheneutralizationandenhancementsites.AndthenitisalsosuggestedthatADEofinfectionislikelytobeinducedbyre-infectionwiththesameserotypeofvirusintypeIIFIPVinfection.Furthermore,U937cellsareconsideredusefulandcanbesubstitutedforthefeli
7、nemacrophagesfordeterminingADEofFIPV-infection.—KEYWORDS:antibody-dependentenhancementofinfection,felineinfectiousperitonitisvirus,felinemacrophage,U937cell.J.Vet.Med.Sci.60(1):49–55,1998Felineinfectiousperitonitis(FIP)isavirus-inducedvaccination[1,22,25–2
8、7,30,34].WepreviouslyreportedchronicallyprogressiveandusuallyfataldiseaseindomesticthatinvitroFIPVinfectionoffelinealveolarmacrophagesandwildFelidae.ThecausativeagentofthisdiseaseisFIPisenhancedbymurinemonocl
