冠狀病毒Coronavirusl論文-2002 Human picornavirus and coronavirus RNA in nasopharynx of children without concurrent respiratory symptoms.pdf

冠狀病毒Coronavirusl論文-2002 Human picornavirus and coronavirus RNA in nasopharynx of children without concurrent respiratory symptoms.pdf

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時間：2020-03-16

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1、JournalofMedicalVirology66:417±420(2002)HumanPicornavirusandCoronavirusRNAinNasopharynxofChildrenWithoutConcurrentRespiratorySymptoms1,22312JohannaNokso-Koivisto,*TeemuJ.Kinnari,PaèiviLindahl,TapaniHovi,andAnnePitkaèranta1EnterovirusLaboratory,DepartmentofMicrobiology,NationalPublicHealthInstit

2、ute(KTL),Helsinki,Finland2DepartmentofOtorhinolaryngology,HelsinkiUniversityCentralHospital,Helsinki,Finland3DepartmentofOphthalmology,HelsinkiUniversityCentralHospital,Helsinki,FinlandTheprevalenceofhumanrhino-,entero-,andRT-PCRassaysdetectviralRNA,remnantsofpastcoronaviruseswasinvestigatedbyR

3、T-PCRininfectionratherthananactivecurrentinfection.nasopharyngealaspiratesfrom107childrenHowever,theadvantageofRT-PCRtechniquesforwithoutconcurrentrespiratorysymptoms.Thecertainimportantrespiratoryviruses,namelyhumanchildrenwereadmittedtothehospitalforelectiverhino-,entero-,andcoronaviruses,can

4、notbeques-surgery.Theparents?lledaquestionnaireabouttionedsinceforthesevirusesnootheravailableprac-theoccurrenceofrespiratorysymptomsfourticalandrapidtechniquesexist[IevenandGoossens,weeksbeforeandtwoweeksafterthesurgery.1997].WhilenasopharyngealcolonizationinhealthyTherateofviraldetectionwas45

5、%inchildrenchildrenwithrespiratorybacterialpathogensisshownwithrelatedpastorrecentrespiratoryinfectionbyseveralstudies[Fadenetal.,1997;Faden,1998;whereas20%ofthesamplestakenfromchild-Bernstein,1999],lessisknownofrespiratoryvirusesinrenwithoutanyrelatedpastorrecentrespiratorythiscontext.Johnston

6、etal.[1993]haveshownthatinfectionswerepositiveforpicornavirusRNA,respiratorypicornavirusescanbefoundin1.4%ofP?0.008.Thirty-one(29%)ofthenasopharyn-healthychildrenbyvirusisolationandin12%byPCRgealaspirateswerepositiveforviralRNA,18%formethod.Thereareonlyafewotherstudiesreportedrhinovirus,and11%f

7、orenterovirusRNA.Cor-wherethepresenceofrespiratoryviruseshasbeenonavirusRNAwasnotfoundinanyofthedemonstratedinhealthychildren[Hornetal.,1979;children.Fifty-?vepercentofthechildrenwithJenningsetal.,1987]andinmostofthepreviousvirus-

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