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1、VeterinaryMicrobiology99(2004)3142PrevalenceoffelinecoronavirustypesIandIIincatswithhistopathologicallyveri?edfelineinfectiousperitonitisVivianeBenetkaa,AnnaKübber-Heissb,JolantaKolodziejeka,NorbertNowotnya,d,MargareteHofmann-Parisotc,KarinM?stla,?aClinicalVirologyGr
2、oup,InstituteofVirology,UniversityofVeterinaryMedicineVienna,Veterin?rplatz1,A-1210Vienna,AustriabInstituteofPathologyandForensicVeterinaryMedicine,UniversityofVeterinaryMedicineVienna,Veterin?rplatz1,A-1210Vienna,AustriacInstituteofMedicalPhysicsandBiostatistics,Uni
3、versityofVeterinaryMedicineVienna,Veterin?rplatz1,A-1210Vienna,AustriadDepartmentofMedicalMicrobiology,FacultyofMedicineandHealthSciences,UnitedArabEmiratesUniversity,P.O.Box17666,AlAin,UnitedArabEmiratesReceived3December2002;receivedinrevisedform1July2003;accepted21
4、July2003AbstractFelinecoronaviruses(FCoV)varywidelyinvirulencecausingaspectrumofclinicalmanifestationsreachingfromsubclinicalcoursetofatalfelineinfectiousperitonitis(FIP).Independentofvirulencevariationstheyareseparatedintotwodifferenttypes,typeI,theoriginalFCoV,andt
5、ypeII,whichiscloselyrelatedtocaninecoronavirus(CCV).TheprevalenceofFCoVtypesinAustriancatpopulationswithoutFIPhasbeensurveyedrecentlyindicatingthattypeIinfectionspredominate.ThedistributionofFCoVtypesincats,whichhadsuccumbedtoFIP,however,wasfairlyunknown.PCRassayshav
6、ebeendevelopedamplifyingpartsofthespikeproteingene.Type-speci?cprimerpairsweredesigned,generatingPCRproductsofdifferentsizes.Atotalof94organpoolsofcatswithhistopathologicallyveri?edFIPwastested.Acleardifferentiationwasachievedin74cats,86%ofthemweretypeIpositive,7%typ
7、eIIpositive,and7%werepositiveforbothtypes.These?ndingsdemonstratethatinFIPcasesFCoVtypeIpredominates,too,nonetheless,in14%ofthecasesFCoVtypeIIwasdetected,suggestingitscausativeinvolvementincasesofFIP.?2003ElsevierB.V.Allrightsreserved.Keywords:Felinecoronavirus(FCoV)
8、;Felineinfectiousperitonitis(FIP);RT-PCR;Paraf?nembeddedtissues(PET);TypesIandII1.Introductionlidae.Thecausativeagent,thefelinecoro
