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1、面北摩業(yè)學(xué)報2O13,22(11):82—88ActaAgriculturaeBoreali~occidentalisSinicadoi:10.7606/j.issn.1004—1389.2013.11.015網(wǎng)絡(luò)出版時間:2013-1卜22網(wǎng)絡(luò)出版地址:http://www.cnki.net/kcms/detail/61.1220.S.20131122.1049.019.html苦豆子根抑菌活性物質(zhì)回流提取工藝張沙沙,馮俊濤,張興(1.西北農(nóng)林科技大學(xué)無公害農(nóng)藥研究服務(wù)中心,陜西楊凌712100;2.陜西省生物農(nóng)藥工程技術(shù)研究中心,陜西楊凌712i00)摘要為建立苦豆子根抑菌活性物
2、質(zhì)的較優(yōu)提取工藝,以提取物對番茄灰霉病菌的抑菌活性為指標(biāo),在單因素試驗的基礎(chǔ)上,通過正交試驗對提取溶劑、液固比等4個工藝條件進(jìn)行考察。結(jié)果表明,其較佳提取工藝條件為以體積分?jǐn)?shù)為75的乙醇溶液為提取溶劑,液固比(溶劑):m(植物材料)一10mL:1g,80℃加熱2h,共提取3次。所得提取物對番茄灰霉病原菌的有效抑制中質(zhì)量濃度(EC。)為185.4mg/L,1000mg/L劑量下對番茄灰霉病的藥效與500mg/L的叫一5O%速克靈可濕性粉劑相當(dāng)。該工藝條件較為簡單,對其放大即可滿足苦豆子殺菌劑產(chǎn)品的工業(yè)化生產(chǎn)要求。關(guān)鍵詞苦豆子;抑菌活性;提取工藝中圖分類號$482.2文獻(xiàn)標(biāo)志碼A文章編號
3、1004—1389(2013)11-0082—07RefluxExtractionTechnologyoftheAntifungalComponentsfromtheRootofSophora口ZDpPcrDZdZHANGShasha。FENGJuntao’andZHANGXing,。(1.Research8LDevelopmentCenterofBiorationalPesticide,NorthwestA8LFUniversity,YanglingShaanxi712100,China2.ShaanxiResearchCenterofBiopesticideEngineering
4、andTechnology,YanglingShaanxi712100,China)AbstractTheobjectiveofthisresearchwastoinvestigatethetechnologyofextractingantifungalsub—stancesfromtherootofSophoraalopecuroides。Thesingle—factorandorthogonalexperimentswereconductedtooptimizeextractionprocess.Theinfluencesofsolvent,liquid—solidratio,e
5、xtracttimesandheattimeonantifungalactivityoftheextractsagainstBotrytiscirereawerediscussed.There—suitssuggestedthebestextractionconditions:ethanolvolumefraction75,liquid—solidratio10:1,heating2hourat8O。C,extracting3times.TheextracthadnoticeableinhibitoryonmycelialgrowthofBotrytiscirereawithEC5。
6、185.4mg/L,andtheextractshowedcertaineffectsonBotrytiscirereaattheconcentrationof1000mg/Lintissuetest,therewasnosignificantdifferenceincomparisonwith5OSumilexWP(500mg/L).Inconclusion,thissimpleandeffectiveextractingmethodhasawideapplicationprospectfordevelopingasplant—sourcefungicide.KeywordsSop
7、horaalopecuroides;Antifungalactivity;Extractiontechnology苦豆子(Sophoraalopecuroides)為豆科槐屬含生物堿外,還含黃酮、有機酸、氨基酸、蛋白質(zhì)和植物,別名苦豆根、苦甘草等,全株味極苦、性寒,多糖等多種物質(zhì)Ⅲ??喽棺硬坏哂辛己玫尼t(yī)用有清熱解毒、祛風(fēng)燥濕、止痛殺蟲等作用,在中國活性,而且具有殺蟲、抑菌和植物生長調(diào)節(jié)等廣譜西北各省(區(qū))均有分布,資源豐富??喽棺又谐r(nóng)用活性]。收稿日期