資源描述：
《黃芪多糖通過(guò)激活線粒體凋亡途徑誘導(dǎo)食管癌EC9706細(xì)胞的凋亡并增加其對(duì)順鉑的敏感性.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫(kù)。
1、中華實(shí)驗(yàn)外科雜志2013年3月第30卷第3期Chin‘JExpSurg·539·March2013，V0l1_30，No．3,·實(shí)驗(yàn)研究·黃芪多糖通過(guò)激活線粒體凋亡途徑誘導(dǎo)食管癌EC9706細(xì)胞的凋亡并增加其對(duì)順鉑的敏感性盧創(chuàng)新羅執(zhí)芬周建煒崔勇霞周云【摘要】目的觀察黃芪多糖、順鉑及黃芪多糖與順鉑聯(lián)合對(duì)EC9706細(xì)胞增殖和凋亡的影響。方法采用噻唑藍(lán)(MTF)比色法及流式細(xì)胞凋亡檢測(cè)技術(shù)檢測(cè)人食管癌EC9706細(xì)胞對(duì)黃芪多糖、順鉑及黃芪多糖聯(lián)合順鉑的藥物敏感性差異；應(yīng)用免疫細(xì)胞化學(xué)技術(shù)檢測(cè)細(xì)胞中B淋巴細(xì)胞／白血病一
2、2(bcl-2)、bc12相關(guān)x蛋白(bax)及核因子一KB(NF—KB)蛋白的表達(dá)。結(jié)果黃芪多糖和順鉑單藥對(duì)食管癌EC9706細(xì)胞的增殖呈濃度依賴性的抑制作用，聯(lián)合組誘導(dǎo)細(xì)胞凋亡率為41．3％，高于單藥黃芪多糖組的18．7％及單藥順鉑組的27．6％(P<0．05)；黃芪多糖能下調(diào)EC9706細(xì)胞中bcl一2、NF'KB蛋白的表達(dá)，灰度值分別為179．22-4-12．47、131．664-18．83，對(duì)照組灰度值分別為142．11±13．35、140．214-14．75，上調(diào)bax蛋白的表達(dá)，其灰度值為185．5
3、4±11．27，對(duì)照組灰度值為199．67±15．43。結(jié)論黃芪多糖通過(guò)激活線粒體凋亡途徑來(lái)促進(jìn)細(xì)胞凋亡，并通過(guò)抑制NF—KB的表達(dá)來(lái)促進(jìn)EC9706細(xì)胞對(duì)順鉑的敏感性?！娟P(guān)鍵詞】食管癌；黃芪多糖；順鉑；脫噬作用；耐藥Mitochondrialapoptosispathwaycontributestopolysaccharideofastragalus-inducedapoptosisofEC9706cellsandenhancestheirsensitivtytocisplatinLUChuang—xin，LU
4、OZhi一尼n，ZHOUJian—wei，CUIYong—xia，ZHOUYun．DepartmentofOneology，People’sHospitalofZhengzho~University，Zhengzhou450003，ChinaCorrespondingauthor：YUNZhou，Email：syzlk2010@yahoo．corn．cn【Abstract】ObjectiveToinvestigatetheantiproliferative，cytotoxic，apoptogenicactivit
5、iesofpoly—saccharideofastragalus(APS)anditssynergisticeffectswithcisplatinininducingapoptosisofesophagealcarcinomacells．MethodsMethylthiazoltetrazolium(MTI")assaywasusedtodetecttheantiproliferativeeffectofAPSandcisplatinonEC9706cells，AnnexinV—fluoresceineisot
6、hiocyanate(FITC)／propidiumio—dide(PI)stainedfluorescence—activatedcellsorter(FACS)wasusedtodetecttheapoptosisrate，andim—munocytochemistrywasusedtoexaminecellularlocalisationandBlymphocytes／leukemia一2(bcl一2)，bcl一2associatedXprotein(bax)，nuclearfactor-KB(NF—KB)
7、proteinlevels．ResultsAPSandcisplatinpro—duceddose—dependentinhibitionofEC9706cellgrowth．TheapoptosisrateinAPS+cisplatingroup(41．3％)washigherthaninAPSgroup(18．7％)orcisplatingroup(27．6％)．APScouldup—regulatethegreyvaluesofBaxprotein(185．54±l1．27)，anddown—reguate
8、thoseofbcl-2(179．22±12．47)andNF—KB(131．66±18．83)proteins．ThegreyValuesbcl-2，NF—KBandBaxincontrolgroupwere142．114-13．35，140．214-14．75and199．674-15．43，respectiely．ConclusionMitochondrialapo
