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1、·508·臨床腫瘤學雜志2014年6月第19卷第6期ChineseClinicalOncology,Jun.2014,Vo1.19,No.6PFKFB3在肝癌中的表達及其臨床意義467000河南平頂山平頂山市第一人民醫(yī)院肝膽外科吳二斌,李莉華,郭子健【摘要】目的探討6一磷酸果糖激酶一2/果糖雙磷酸酶一2同工酶3(PFKFB3)在肝細胞癌(HCC)組織中的表達及其與臨床病理特征的關(guān)系。方法采用逆轉(zhuǎn)錄一聚合酶鏈反應(yīng)(RT—PCR)檢測PFKFB3在42例HCC組織及其對應(yīng)癌旁組織中的表達情況,并研究其與臨床病理參數(shù)(性別、年齡、腫瘤直徑、淋巴轉(zhuǎn)移、靜脈癌栓、治療前甲胎蛋白、分化程度及肝內(nèi)轉(zhuǎn)
2、移)間的關(guān)系,采用Kaplan—Meier法分析PFKFB3mRNA表達對HCC患者生存的影響。結(jié)果HCC和癌旁組織中PFKFB3mRNA的陽性表達率分別為73.81%(31/42)和52.38%(22/42),相對表達量為1.075±0.171和0.877±0.451,HCC組織中PFKFB3mRNA的陽性表達率及相對表達量均高于癌旁組織(P<0.05);PFKFB3mRNA的表達與腫瘤TNM分期、分化程度、肝內(nèi)轉(zhuǎn)移及總生存期有關(guān)(P<0.05)。結(jié)論PFKFB3在HCC的發(fā)生發(fā)展中發(fā)揮作用,且影響肝癌的分化、轉(zhuǎn)移并提示肝癌患者預(yù)后不佳;可作為評估HCC惡性程度的生物學指標?!娟P(guān)鍵詞
3、】6一磷酸果糖激酶一2/果糖雙磷酸酶一2同工酶3;肝細胞癌;逆轉(zhuǎn)錄一聚合酶鏈反應(yīng);預(yù)后中圖分類號:R735.7文獻標識碼:A文章編號:1009—0460(2014)06—0508—04ExpressionofPFKFB3inhumanprimaryhepatocellularcarcinomaanditsclinicalsignificanceErbin,LILihua,GUOZ~ian.DepartmentofHepatobiliarySurgery,theFirstPeople'sHospitalofP一dingshan,Pingdingshan467000,China【Abstr
4、act】ObjectiveToinvestigatetheexpressionof6-phosphofructo一2一kinase/fructose一2,6-bisphosphatase(PFKFB3)inhepatocellularcarcinoma(HCC)anditsclinicalsignificance.MethodsTheexpressionsofPFKFB3mRNAoftissuesectionsfrom42HCCandpairedadjacenttissuesweredetectedbyRT—PCR.ThentherelationshipsbetweenPFKFB3ex
5、pressionandclinicopathologicalvariables(gender,age,tumorsize,lymphaticmetastasis,cancerembolusinvein,AFPlevelbeforetreatment,differentiationandme—tastasisinliver)wereanalyzed.Meanwhile,thedifferenceofoverallsurvivalofpatientswithdifferentexpressionofPFKFB3werestud—iedbysurvivalanalysisofKaplan—m
6、eier.ResultsThepositiveexpressionrateandrelativelevelofPFKFB3inHCCtissuewere73.81%(31/42)and1.075+0.171,higherthan52.38%(22/42)and0.877~0.451inadjacenttissueswithsignificantdifference(P<0.05).ThePFKFB3expressionisrelatedwithTNMstage,degreeofdifferentiation,intrahepaticmetastasisandoverallsurviva
7、l(P<0.05).ConclusionPFKFB3wererelatedtocarcinogenesis,developmentandmetastasisofHCC.TheexpressionofPFKFB3caninfluencethedifferentiation,metastasisandprognosisofHCC,indicatingitmaybeusedasbiologicalindicatorstoevaluatethedegr