芬太尼聯(lián)合丙泊酚對(duì)大鼠肺缺血再灌注損傷的保護(hù)作用-論文.pdf

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1、·論著·中國(guó)現(xiàn)代醫(yī)生2014年5月第52卷第15期芬太尼聯(lián)合丙泊酚對(duì)大鼠肺缺血再灌注損傷的保護(hù)作用溫文郭光偉黃志剛孫國(guó)棟李曉輝山西醫(yī)科大學(xué)第二醫(yī)院心胸外科，山西太原030001[摘要】目的探討芬太尼聯(lián)合丙泊酚作用于大鼠肺缺血再灌注損傷(I／R)模型后，對(duì)核因子一KB(nuclearfactor—KB)表達(dá)的影響及其機(jī)制。方法將40只雄性Wistar大鼠隨機(jī)分為5組(n=8)：假手術(shù)組(S組)、缺血再灌注損傷組(I／R組)、芬太尼干預(yù)組(F組)，丙泊酚干預(yù)組(P組)，芬太尼聯(lián)合丙泊酚干預(yù)組(F+P組)。對(duì)大鼠動(dòng)脈血進(jìn)行血?dú)夥治觯粰z測(cè)大鼠肺組織濕

2、／干(w／D)值；對(duì)大鼠肺組織進(jìn)行大體形態(tài)學(xué)及HE染色后鏡下病理學(xué)觀察，采用免疫組化法測(cè)定大鼠肺組織NF—KB表達(dá)水平的變化。結(jié)果與I／R組比較，F(xiàn)組、P組以及F+P組大鼠肺組織核因子一KB表達(dá)明顯降低，差異有統(tǒng)計(jì)學(xué)意義(P

3、構(gòu)較完整性，減輕肺間質(zhì)及肺泡腔滲出、水腫肺組織保護(hù)方面均較I／R組明顯減輕。結(jié)論芬太尼聯(lián)合丙泊酚干預(yù)可顯著減輕大鼠肺缺血再灌注損傷，可能是芬太尼聯(lián)合丙泊酚能降低大鼠肺組織NF—KB水平有關(guān)。【關(guān)鍵詞】芬太尼；丙泊酚；核因子一KB；缺血再灌注損傷【中圖分類號(hào)】R614【文獻(xiàn)標(biāo)識(shí)碼】A【文章編號(hào)】1673—9701(2014)15—0010—04Protectiveefectsoffentanylcombinedwithpropofolonpulmonaryis-chemiareperfusioninjuryinratsWENWenGUOGuan

4、gweiHUANGZhigangSUNGuodongLIXiaohuiDepartmentofCardiothoracicSurgery，theSecondAffiliatedHospitalofShanxiMedicalUniversity，Taiyuan030001，China【Abstract】0bjectiveTostudytheexpressionofnuclearfactor-KB(NF—KB)inthelungandplasmaseparatelyandex—plorethepossiblemechanismsoffentany

5、lcombinewithpropofolonlungischemia—reperfusioninjuryofrats．MethodsThe40Wistarmaleratsweighing220—·270gweredividedrandomlyintofivegroupswhichwerepretreateddifferent—ly．ThevaluesofNF-KBinrats’lungwasdetectedbyusingimmunohistochemicalmethod，thelungtissueobservedinvitroandunder

6、themicroscopeafterHEstaining，therats’arterialoxygenpressureandthelungtissueW／Dratioweremeasuredineachgroup．ResultsComparedwiththeI／Rgroup，theFgroup，thePgroup，andtheF+Pgroupall~llowedthesametrend：theNF-KBexpressionlevelswereobviouslylowerfP<0．01)。thevaluesofPaOzwereobviously

7、higher(P<0．O1)，andtheW／Dratioswerelower(P<0．o1)．ConclusionFentanylcombinedwithpropofolcansignificant—lyreducelungischemia-reperfusioninjury，itmaybethatfentanylcombinedwithpropofolcanreducethelevelofNF—KBinrats’lung．【Keywords】Fentanyl；Propofol；Nuclearfaetor-KB；Ischemiareperf

8、usioninjury缺血再灌注常發(fā)生在組織器官缺血后恢復(fù)血供．發(fā)現(xiàn)，芬太尼及丙泊酚可抑制NF—KB的過(guò)度產(chǎn)生，從如休克時(shí)微循環(huán)的疏通、冠狀動(dòng)脈痙攣的緩解、溶栓而起