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1、中華實(shí)驗(yàn)外科雜志2014年3月第31卷第3期ChinJExpSurg，March2014，V．．·6l3··實(shí)驗(yàn)研究·包芯結(jié)構(gòu)骨支架修復(fù)兔橈骨缺損王闖建張春霖吳學(xué)建朱旭李瑩嚴(yán)旭【摘要】目的采用骨髓基質(zhì)干細(xì)胞(BMSCs)復(fù)合包芯結(jié)構(gòu)骨支架修復(fù)兔橈骨缺損。方法成骨誘導(dǎo)培養(yǎng)3周的BMSCs與包芯結(jié)構(gòu)骨支架復(fù)合構(gòu)建細(xì)胞支架復(fù)合體(A組)，BMSCs／聚乳酸羥基乙酸(PLGA)／[3磷酸三鈣([3-TCP)復(fù)合體(B組)、空白缺損(C組)作為對(duì)照。采用兩因素隨機(jī)區(qū)組設(shè)計(jì)植入36只兔(72側(cè))橈骨1．5cm缺損部位，術(shù)后l2、24、48周取材，進(jìn)行相關(guān)分析。結(jié)果大體觀察、
2、影像學(xué)、組織學(xué)檢測(cè)示術(shù)后12周，A組骨缺損兩斷端骨性相連，24周A組支架內(nèi)部大量新生骨生成，髓腔部分再通，48周A組髓腔再通，骨缺損完全修復(fù)，Lane—Sandhu評(píng)分示A組(5、8、12分)骨缺損修復(fù)效果明顯好于B組(1、4、6分)、C組(0、0、1分)(P<0．05，n：4)。結(jié)論BMSCs／包芯結(jié)構(gòu)骨支架復(fù)合體可修復(fù)兔橈骨1．5cm缺損?！娟P(guān)鍵詞】包芯結(jié)構(gòu)骨支架；骨髓基質(zhì)干細(xì)胞；大段骨缺損；骨組織工程Repairofrabbitsegmentalradialdefectbyusingbonemesenchymalstemcellsintothewrappe
3、d-corestructurecompositescafoldWangChuangfian，ZhangChunlin，WuXuejian，ZhuXu，LiYing，YanXu．DepartmentofOrthopaedics，theFirstAffiliatedHospitalofZhengzhouUniversity，Zheng—zhou450052，ChinaCorrespondingauthor：WangChuangfian，Email：wangchuang20051983@126．corn【Abstract】ObjectiveTorepairsegmen
4、talradialdefectbyusingbonemesenchyma!stemcells(BMSCs)culturedinvitrofor3weeksunderosteogenicmediumintoaporouswrapped—corestructureCOB—positescaffoldcomposedofpoly(1actic—co—glycolicacid)／[3一tricalciumph0sphate(PLGA／13一TCP)skeletonwrappedwithtypeIcollagen．Methods1．5cmsegmentalradialde
5、fectswerecreatedbilaterallyin36rab-bits，thenBMSCsincombinationwithwrapped—corestructurecompositescaffold(groupA)andBMSCsincombinationwithPLGA／[3一TCPskeleton(groupB)weretransplantedintotheuntreatedradialdefeetasblankcontrolgroup(groupC)．12，24and48weeksafterimplantation，12animalsweresa
6、crificedandtheconstructswereharvestedandanalyzed．ResultsIngroupA，synosteosisformedbetweenthetwoendsofthecritical—sizebonedefect12weekspostoperation：boneintegritywasalmostfinishedandbonemarrowstartedatrendofrecanalization24weekspostoperation；bonedefectrepairwascompletedandbonemarrowwa
7、srecanalized48weekslater．AscomparedwithgroupB(1，4，6points)，andgroupC(0，0，1point)，Lane—SandhuindicatedthatthenewboneformationingroupA(5，8，and12points)wassignifi—cantlyincreased(P<0．05，n=4)．ConclusionThecombinationofBMSCsandcore—sheathstructurecompositescaffoldexhibitsafavorableosteoge
8、nesis，biocom
