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1、2014.06,35(3)中國(guó)煙草科學(xué)ChineseTobaccoScience61煙草青枯病生防菌YH一22抗病機(jī)制的初步研究楊歡,余君,王昌軍,李進(jìn)平,施河麗3,譚軍。,李錫宏,王瑞,徐迪紅,陳守文(1.華中農(nóng)業(yè)大學(xué)農(nóng)業(yè)微生物國(guó)家重點(diǎn)實(shí)驗(yàn)室,武漢430070;2.湖北省煙草科研所,武漢430030;3.湖北省煙草公司恩施州公司科技中心,湖北恩施445000)摘要:通過(guò)菌株生態(tài)定殖以及抗病物質(zhì)分析,初步探究解淀粉芽胞桿菌(Bacillusamyloliquefaciens)YH·22抗青枯病(茄科雷爾氏菌所致)的機(jī)制。采用利福平抗性誘導(dǎo),獲得抗400gg
2、/mL的抗性菌株以利于定殖研究。盆栽試驗(yàn)結(jié)果表明,YH-22先在煙草根際土壤定殖,然后依次進(jìn)入根部、莖部。對(duì)YH一22菌株發(fā)酵上清液進(jìn)行理化性質(zhì)分析,發(fā)現(xiàn)抗茄科雷爾氏菌活性物質(zhì)不溶于氯仿和乙酸乙酯,能溶于甲醇,具有一定的耐熱性,能耐受胃蛋白酶和紫外線,具有排油和使液滴坍塌的特性。綜上所述,生防菌YH一22在煙草根際土壤、根、莖均具有一定的定殖能力,其抗菌物質(zhì)可能為蛋白質(zhì)和脂肽的混合物。關(guān)鍵詞:解淀粉芽胞桿菌;茄科雷爾氏菌;定殖;抗菌物質(zhì);理化性質(zhì)中圖分類號(hào):$572.08文章編號(hào):1007—5119(2014)030061—06DOI:10.13496~.
3、issn.1007—5119.2014.03.013StudyonResistanceMechanismofBio—controlStrainBacillusamylofiquefaciensYH一22againstTobaccoWiltYANGHuan,YUJun2,WANGChangjun2,LIJinping,SHIHeli,TANJun,LIXihong,WANGRui,XUDihong,CHENShouwen(1.StateKeyLaboratoryofAgriculturalMicrobiology,HuazhongAgriculturalUn
4、iversity,Wuhan430070,China;2.HubeiTobaccoResearchInstitute,Wuhan430030,China;3.EnshiTobaccoCompanyofHubeiProvince,Enshi,Hubei445000,China)Abstract:Throughtheanalysisofecologica1colonizationandantimicrobialsubstancesofBacillusamyloliquefaciensYH.22.theresistancemechanismofYH.22agai
5、nstbacterialwiltwhichwascausedbyRalstoniaSOlanacearumwaspreliminarilyinvestigated.ThetolerancedosageofYH-22resistancestrainagainstrifampicinreached400gg/mLbyresistanceinductionwithrifampicin.TheresultsofpotexperimentsshowedthatYH一22resistancestrainfirstlycolonizedontherhizospheres
6、oil.Then.itintrudedintotherootandstemsuccessively.Theantimicrobialsubstancesinbrothcouldbedissolvedbymethano1.butnotbychloroformandethylacetateTheycouldmaintainhighstabilityundertheconditionsofheattreatment,proteasehydrolysisandUltraviolet.Moreover,theyhadthecharacterofoildisplace
7、mentanddropletcollapse.Inconclusion,strainYH一22hascolonizationabilityinrhizospheresoilaswellastobaccotissue.TheantibacterialcomponentsofstrainYH一22mayconsistofproteinsandlipopeptides.Keywords:Bacillusamylofiquefaciens;RalstoniaSOlanacearum;colonization;antibioticsubstance;physicoc
8、hemicalcharacteristic煙草是一種重要的農(nóng)業(yè)經(jīng)濟(jì)