PIWILl蛋白與DICER酶在肝細胞癌中的表達及意義-論文.pdf

《PIWILl蛋白與DICER酶在肝細胞癌中的表達及意義-論文.pdf》由會員上傳分享，免費在線閱讀，更多相關內(nèi)容在應用文檔-天天文庫。

1、·9O8·中華肝膽外科雜志2013年12月第19卷第12期ChinJHepatobiliarySurg，December2013，Vo1．19，No．12·論著·PIWIL1蛋白與DICER酶在肝細胞癌中的表達及意義范正軍孫濤楊飛龍程波彭飛周闖【摘要】目的探討P1wII1蛋白與DICER酶在肝細胞癌組織中的表達及臨床意義。方法免疫組化檢測47例肝細胞癌標本中PIWIL1與DICER的表達。蛋白印跡法檢測31例肝細胞癌組織中PIWIL1與DICER的表達。分析PIWII1與DICER表達及與臨床特征的關系。結果PIWlI1在癌組織中高表達，在正常肝組織中低表達

2、(P

3、carcinomaFANZheng—un，SUNTa0，yANGFei—long，CHENGBo，PENGFei，ZH0UChuang．eFirstAffiliatedHospital0fZhengzhouUniversity，Zhengzhou450052，ChinaC0rre聲。ngauthor：FANZheng—Un，Email：droider@163．com[Abstract]ObjectiveToexploretheexpressionofPIWIL1proteinandDICERenzymeinhepatocellularcarcinoma(HC

4、C)andtheirsignificance．MethodsImmunohistochemica1methodwasusedtodetecttheexpressionofPIWIL1andDICERin47casesofHCCandtheadjacentHCCtissues．WesternblotmethodwasusedtOdetecttheexpressionofPIWIL1andDICERin31casesoffreshHCCtissuesandtheiradjacentHCCtissues．TherelationshipbetweenPIWIL1an

5、dDICERandtheirrelationshipswereanalysedwithclinica1features．12casesofnorma1livertissueswereusedascontrolgroup．ResultsTheexpressionofPIWIL1washighinHCCbutlOWinnormal1ivertissues(P

6、velycorrelatedwithinvasiontoadjacenttissuesandhistologicaldiffer—entiation(Pd0．O5)．TheexpressionofDICERwasnegativelycorrelatedwithinvasiontotheadacenttissuesandhistologica1differentiation(Pd0．05)．ThereW38anegativecorrelationbetweenPIWIIlandDICER(P<0．05)．ConclusionsHighexpressionofP

7、IWII1andlow／missingexpressionofDICERwasrelatedtopathologicaldifferentiationandinvasionofadjacenttissues．[Keywords]Hepatocel1ularcarcinoma；Piwilikeprotein；Ribonuclease1I，DICER肝細胞癌(hepatoce11ularcarcinoma，HCC)具原(Piwi-likeprotein1，PIWILl／H1wI)為新發(fā)現(xiàn)有發(fā)病率高、難以早期發(fā)現(xiàn)、病死率高等特點。我國的惡性腫瘤基因蛋白。PIWII

8、1與多種腫瘤的發(fā)生每年因肝細胞癌死亡的人數(shù)在惡性腫瘤