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1、中華麻醉學(xué)雜志2013年9月第33卷第9期ChinJAnesthesiol，September2013，Vo1．33，No．9ll3l·重癥醫(yī)學(xué)·異丙酚對(duì)急性肝衰竭小鼠肝損傷的影響李小剛景桂霞周榮勝朱宇麟李鋒【摘要】目的評(píng)價(jià)異丙酚對(duì)急性肝衰竭小鼠肝損傷的影響。方法成年雄性ICR小鼠80只，1月齡，體重2O～25g，采用隨機(jī)數(shù)字表法分為3組(n=20)：對(duì)照組(I組)、急性肝衰竭組(Ⅱ組)和異丙酚組(Ⅲ組)。采用腹腔注射D．氨基半乳糖和LPS的方法制備小鼠急性肝衰竭模型，Ⅲ組腹腔注射D一氨基半乳糖和LPS后6h內(nèi)，每隔1h尾靜脈注射異丙酚5ms／kg，其余組相應(yīng)時(shí)點(diǎn)注射等容量生理鹽水。腹腔注
2、射D．氨基半乳糖和LPS后1、3和6h(T)時(shí)采尾靜脈血樣，測(cè)定血清AST和ALT活性，ELISA法測(cè)定血清TNF-a、IL-I~和IL-IO濃度；腹腔注射D一氨基半乳糖和LPS后12h觀察小鼠生存情況，采用頸椎脫臼法處死存活小鼠，取肝組織觀察病理學(xué)結(jié)果。結(jié)果與I組比較，Ⅱ組和Ⅲ組各時(shí)點(diǎn)血清AST和AIJT活性、Ⅱ組rI’I．時(shí)血清TNF—a濃度、各時(shí)點(diǎn)血清IL-1~3和IL-IO濃度，Ⅲ組rI’J時(shí)血清TNF．a(chǎn)濃度、T2．，時(shí)血清IL-lf]和IL-IO濃度升高(P<0．05)；與Ⅱ組比較，Ⅲ組各時(shí)點(diǎn)血清AST和ALT活性、，r1．：時(shí)血清TNF—a濃度、rr2．，時(shí)血清IL-lf]和
3、IL-IO濃度降低，腹腔注射后12h小鼠生存率升高(P<0．05)。Ⅱ組和Ⅲ組肝組織病理學(xué)損傷程度依次減輕。結(jié)論異丙酚可通過(guò)抑制炎性反應(yīng)減輕急性肝衰竭小鼠的肝損傷?！娟P(guān)鍵詞】二異丙酚；蛋白酶抑制劑；肝功能衰竭，急性EfectofpropofolonHverinjuryinmicewithacuteHverfailureL／Xiao—gⅡJ曙，JINGGui—xia，ZHOURong-sheng，ZHUYu-lin，12Feng．DepartmentofAnesthesiology，F(xiàn)imtAJ~liatedHospitalofMedicalCollege，Xi’anJiaotongUnwe
4、rsity，Xian710061，ChinaCorrespondingauthor：12Xiao—glⅡng，Email：~g945945@sina．eta【Abstract】ObjectiveToevaluatetheefectofpropofolonliverinjuryinmicewithacuteliverfailure(ALF)．MethodsEightyadultmaleICRmice，aged1months，weighing20—25g，wererandomlydividedinto3groups(n=20each)usingarandomnumbertable：contro
5、lgroup(groupI)，ALFgroup(groupⅡ)，andALF+propofolgroup(groupm)．ALFmodelwasestablishedwithintra—peritonealD—galaetosamine(D—GaIN)andli—popolysaecharide(LPS)．Pmpofol5mg／kgwasi~ectedviathetailveinevery1hwithin6hafterinjectionofD—GaINandLPSingrouplI，whiletheequalvolumeofnormalsalinewasgiveninsteadintheo
6、thergroups．Venousbloodsamplesweretakenfromthetailveinat1，3and6hafterinjectionofD—GaINandLPS(Tl-])todetecttheactivitiesofserumaspartateaminotrans~rase(AST)andalanineaminotransferase(ALT)andserumtumornecrosisfactor-alpha(TNF—d)，interleukin一1B(IL-t#)andIL-10concentrations(byILISA)．Thesurvivalwithin12
7、hafterinjectionofD—GaINandLPSwasobservedandthesurvivalrateswerecalculated．Themiceweresacrificedandliv—erswereremovedformicroscopicexaminationofpathologicchanges．ResultsComparedwithgroupI，theactivi—tiesofASTandAU’
