司來吉蘭對帕金森病模型大鼠黑質紋狀體TH及GDNF表達的影響.pdf

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1、154TianjinMedJ，F(xiàn)ebruary2015，Vo1．43No．2司來吉蘭對帕金森病模型大鼠黑質紋狀體TH及GDNF表達的影響呂超男，毛文靜，馬原源，劉斌，張晉霞，孫靜，成曉華，李世英摘要：目的觀察司來吉蘭對帕金森病(PD)模型大鼠黑質紋狀體內酪氨酸羥化酶(TH)及膠質細胞源性神經(jīng)營養(yǎng)因子(GDNF)表達的影響，探討司來吉蘭對多巴胺能神經(jīng)元的保護作用及機制。方法72只健康雄性sD大鼠隨機分為對照組、模型組和司來吉蘭組，每組均設4d和8d2個亞組，各l2只。模型組和司來吉蘭組采用頸背部皮下注射魚藤酮制備PD模型，對照組

2、皮下注射等體積葵花油。之后司來吉蘭組每日灌胃咪多吡0．5mg／kg，模型組和對照組每13灌胃等體積生理鹽水，4d和8d組分別連續(xù)灌胃4d和8d。采用免疫組化法和Westernblotting法檢測黑質紋狀體TH和GDNF表達水平。結果免疫組化法和Westernblotting法檢測結果均顯示，對照組大鼠黑質紋狀體可見多量TH陽性細胞表達和少量GDNF陽性細胞表達，8d和4d組差異均無統(tǒng)計學意義。模型組TH和GDNF陽性細胞表達均明顯低于對照組(均P<0．05)，8d和4d組差異均元統(tǒng)計學意義。司來吉蘭組TH陽性細胞表達低于對照

3、組而高于模型組，GDNF陽性細胞表達高于對照組和模型組(均P

4、ntianigraandstriatuminParkinson’sdiseasemodeIinratLYUChaonan，MAOWenjing，MAYuanyuan，LIUBin，ZHANGJinxia，SUNJing，CHENGXiaohua，LIShiyingFirstDepartmentofNeurology,theAffiliatedHospitalofHebeiUnitedUniversity,Tangshan063000,ChinaCorrespondingAuthorE—mail：liubintsh@126．co

5、mAbstract：0bjectiveToobservetheeffectsofEldeprylonexpressionsoftyrosinehydroxylase(TH)andglialcellline—derivedneurotrophicfactor(GDNF)insubstantianigraandstriatuminParkinson’sdisease(PD)andtoexploretheprotectivemechanismofEldeprylondopaminergicneuron．MethodsHealthym

6、aleSprague—Dawley(SD)rats(n=72)wererandomlydividedintocontrolgroup，modelgroupandEldeprylgroup(n=24ineachgroup)．Eachgroupwasdividedrandom—lyinto2subgroupsas4daytreatmentgroupand8daytreatmentgroup(n=12ineachsubgrop)．Pakinson’sdiseasemodelwasestablishedbyinjectingroten

7、onesubcutaneouslybacktheneck，ratsinthecontrolgroupwerei~ectedwithanequalvol—umeofsunfloweroilsubcutaneouslyatthesamelocation．RatsintheEldeprylgroupwerethengivenEldepryl0．5mg·kg—in—tragastrieallyeverydayfor4or8consecutivedaysandratsinmodelgroupandcontrolgroupweregive

8、nanequalvolumeofsalineinstead．TheexpressionofTHandGDNFinsubstantianigraandstriatumweredetectedbyimmunohistochemistryandWesternblotting．Res