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1、南方農(nóng)業(yè)學(xué)報JOURNALOFSOUTHERNAGRICULTURE2013,44(7):1210—1214ISSN2095—1191;CODENNNXAABhttp://www.nfhyxb.cnDOI:10.3969/j:issn.2095—1191.2013.7.1210宿主濕度環(huán)境對球孢白僵菌致病力的影響唐亮,石美寧+,朱方容,潘志新,黃旭華,胡文娟,黃深惠(廣西蠶業(yè)技術(shù)推廣總站/廣西蠶業(yè)科學(xué)研究院,南寧530007)摘要:【目的】研究宿主濕度環(huán)境對球孢白僵菌致病力的影響,為采取適當(dāng)防治措施以減少白僵病對蠶繭生產(chǎn)的危害提供參考依據(jù)?!痉椒ā恳詮V
2、西象州蠶區(qū)球孢白僵菌感染家蠶獲得的白僵病蠶尸體,觀察不同濕度環(huán)境中白僵病蠶尸體形態(tài)的變化情況,并每隔15d進(jìn)行一次分生孢子致病力測試?!窘Y(jié)果】在25℃下的3種濕度環(huán)境(60%RH、75%RH和90%RH)中,白僵病蠶尸體在同一宿存時間內(nèi),球孢白僵菌活力與宿存濕度關(guān)系密切,在60%RH中宿存的蠶體保存較完好,60d后仍然可見淡黃白色的分生孢子,尸體質(zhì)硬;隨濕度增加,宿存病原活力逐漸降低。3種濕度條件下球孢白僵菌在2齡病蠶尸體中宿存30d內(nèi),對家蠶仍有很強的致病性,其半致死時間(L氏)分別為2.915、3.071和5.222d;在60%~75%RH條件下,
3、宿存45~60d的球孢白僵菌對家蠶仍具有一定的致病力;而在90%RH條件下,宿存45d后球孢白僵菌分生孢子數(shù)量急劇下降,無法測定其致病力。【結(jié)論】球孢白僵菌在病蠶體中宿存60d內(nèi)對家蠶的致死率仍然很高,因此及時清除病蠶尸體并使尸體病原性滅活,可降低白僵菌的二次感染。關(guān)鍵詞:家蠶;球孢白僵菌;致病性;半致死時間;宿存中圖分類號:$884.31文獻(xiàn)標(biāo)志碼:A文章編號:2095—1191(2013)07—1210—05Effectsofhost’SenvironmentalhumidityonpathogenicityofBeauveriabassianaT
4、ANGLiang,SHIMei-ning4,ZHUFang-rong,PANZhi-xin,HUANGXu-hua,HUWen-juan,HUANGShen-hui(GuangxiSericuhureTechnologyPromotionStation/GuangxiAcademyofSericuhureSciences,Nanning530007,China)Abstract:【Objective]Effectsofhost’SenvironmentalhumidityonpathogenicityofBeauveriabassianaduringw
5、hitemuscardineepidemicwerestudiedtoprovidereferencesforadoptingpropertreatingandcontrollingmeasurestoeliminatetheinfluenceofwhitemuscardineonsilkwormproduction.【Method】WhitemuscardinesilkwormbodiescollectedfromXiangzhouCountyofGuangxiwereusedtoinvestigatetheirchangesindifferenth
6、umidenvironmentandpathogenicityevery15days.【Result】Whitemuscardinesilkwormbodieswereobservedattemperatureof25℃in60%,75%and90%RHenvironment.respectively.’rheresultsshowedthattherewasacloserelationshipbetweenhumidityandsurvivalabilityofBeauveriabassiana.Instorageenvironmentof60%RH
7、.silkwormbodiesremainedintactandhardwithyellowishwhiteconidiumclearlyseenon60d.Thepathogenactivitywentdownprogressivelyatincreasinghumidity.ThosethreetreatmentsallhadstrongpathogenicityofBeauveriabassianato2甜-instarofsilkwormduringthefirst30days.TheirIJT卯was2.915,3.071and5.222d,
8、respectively.At60%一75%RH,Beauveriabassianastill