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1、安徽農(nóng)業(yè)科學(xué)，JournalofAnhuiAgri．Sci．2012，40(28)：13799—13801，13803責(zé)任編輯朱瓊瓊責(zé)任校對(duì)李巖不同球孢白僵菌菌株生物學(xué)特性與其對(duì)家蠶致病力的關(guān)系駱海玉，鄧業(yè)成，廖永梅，李瑞鈺(廣西師范大學(xué)生命科學(xué)學(xué)院，珍稀瀕危動(dòng)植物生態(tài)與環(huán)境保護(hù)省部共建教育部重點(diǎn)實(shí)驗(yàn)室，廣西桂林541004)摘要[目的]探討球孢白僵菌菌株的生物學(xué)特性與其對(duì)家蠶致病力的關(guān)系，為家蠶白僵病的防治提供科學(xué)依據(jù)。[方法]從我國(guó)各地收集6個(gè)白僵菌殺蟲(chóng)劑，并從廣西養(yǎng)蠶區(qū)采集3個(gè)僵蠶樣品，對(duì)從中分離純化的菌株進(jìn)行形態(tài)觀察和分子鑒定，同時(shí)對(duì)家蠶進(jìn)行致病力測(cè)定，
2、比較各菌株茵落直徑、產(chǎn)孢量、胞外蛋白酶產(chǎn)生水平等生物學(xué)特征指標(biāo)的差異。[結(jié)果]獲得的9個(gè)分離菌株均為球孢白僵茵(BeauveriabassianaVuillemin)；各菌株對(duì)家蠶都有較強(qiáng)致病力，且致病力有一定差異；各菌株菌落生長(zhǎng)直徑、產(chǎn)孢量及胞外蛋白酶活性均存在較大差異，并與其對(duì)家蠶的致病力之間存在相關(guān)關(guān)系。[結(jié)論]各菌株產(chǎn)孢量和胞外蛋白酶活性與其對(duì)家蠶的致病力之間存在顯著正相關(guān)。關(guān)鍵詞球孢白僵菌；生物學(xué)特性；致病力；家蠶中圖分類號(hào)S881．2文獻(xiàn)標(biāo)識(shí)碼A文章編號(hào)0517—6611(2012)28—13799—03RelationshipbetweenBiol
3、ogicalCharacteristjcsofBeauvenabassiana(Bals．)VuillandPathogenicitytoBombyxmoriL．LUOHai-yuetal(CollegeofLifeScience，GuangxiNormalUniversity；KeyLaboratoryofEcologyofRareandEndangeredSpeciesandEnvironmentalProtection，Guilin，Guangxi541004)Abstractj0biectivefThisstudywastoinvestigatether
4、elationshipbetweenbiologicalcharacteristicsofBeauveriabassiana(Bals．)Vuil1andpathogenicityofBombyxmoriL．，withtheaimtoprovidescientificbasisforthecontrolofwhitemuseardineinBombyxmoriL．．[Meth—od]Thestrainsisolatedandpurifiedfromthe6Beauveriabassianabioeontrolagentsfromalloverthecountry
5、andthe3whitemuseardinesilk—WO1TIIScollectedfromGuangxiprovincialsilkwormrearingareaswereidentifiedbythemorphologicalobservationandmolecularbiologytechnol—ogy．ThepathogenicityofsilkwormstoB．bassainawasdetermined，andthebiologicalcharacteristicssuchasgrowthdiameter，sporeproduc—tionamoun
6、tandtheextracellularproteaseactivityofthedifferentB．bassianastrainswerecompared．[Result]Theisolated9strainswereallB．bassaina(Bals．)Vuillemin，andallstrainshadhighpathogenicitytosilkworn)，butwithdifferentpathogenicities．Thegrowthdiameter，sporeproductionamountandextracellularproteaseact
7、ivityofdifferentB．bassianastrainswerealsodifferent，andshowedcorrelationwiththepatheogenieitytosilkworms．[Conclusion]B．bassianasporesproductionanlountandexocellularproteaseactivityhadsignificantpositivecot—relationwiththeirpathogenicitytosilkworm．KeywordsBeauveriabassiana(Bals．)Vuill；
8、Biologicalch
