資源描述：
《玫煙色棒束孢的生物學(xué)特性與其對(duì)小菜蛾致病力的相關(guān)性.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、第37卷第3期植物保護(hù)學(xué)報(bào)Vo.l37No.32010年6月ACTAPHYTOPHYLACICASINICAJune2010玫煙色棒束孢的生物學(xué)特性與其對(duì)小菜蛾致病力的相關(guān)性121*1雷妍圓呂利華何余容梁淑儀(1.華南農(nóng)業(yè)大學(xué)資源環(huán)境學(xué)院,廣州510642;2.廣東省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所,廣州510640)摘要:測(cè)定了玫煙色棒束孢原始親本菌株P(guān)FCFO、退化親本菌株P(guān)FCFD和單孢分離子菌株P(guān)FCFD58、PFCFD20、PFCFD50的菌落生長(zhǎng)速率、產(chǎn)孢量、孢子萌發(fā)率和胞外蛋白酶水平等生物學(xué)指標(biāo)以及各菌株對(duì)小菜蛾2齡幼蟲的校正死亡率。結(jié)果表明
2、,菌株P(guān)FCFO的生長(zhǎng)速率呈先快后慢7的趨勢(shì),產(chǎn)孢量為2.710個(gè)/mL,孢子萌發(fā)率為62.59%,胞外蛋白酶水平為1.16,對(duì)小菜蛾2齡幼蟲校正死亡率達(dá)97.74%。而菌株P(guān)FCFD、PFCFD20、PFCFD50和PFCFD58間平均生長(zhǎng)速77率和孢子萌發(fā)率無(wú)顯著差異,產(chǎn)孢量變化范圍為1.0510~2.3410個(gè)/mL,胞外蛋白酶水平變化范圍為1.02~1.07,各生物學(xué)指標(biāo)及其對(duì)小菜蛾2齡幼蟲的校正死亡率(19.86%~51.44%)都顯著低于菌株P(guān)FCFO。相關(guān)性分析結(jié)果顯示,玫煙色棒束孢對(duì)小菜蛾的致病力與菌株生長(zhǎng)初期的生長(zhǎng)速率、產(chǎn)孢量、孢子萌發(fā)率和胞外蛋白
3、酶水平呈極顯著的正相關(guān)關(guān)系,與菌株生長(zhǎng)后期的生長(zhǎng)速率呈極顯著負(fù)相關(guān)關(guān)系。關(guān)鍵詞:玫煙色棒束孢;小菜蛾;生物學(xué)特性;致病力;相關(guān)性CorrelationbetweenbiologicalcharacteristicsofIsariafumosoroseaanditspathogenicityagainstPlutellaxylostella121*1LeiYanyuanLLihuaHeYurongLiangShuyi(1.CollegeofResourcesandEnvironment,SouthChinaAgriculturalUniversity,Guangzhou5106
4、42,GuangdongProvince,China;2.PlantProtectionResearchInstitute,GuangdongAcademyofAgriculturalSciences,Guangzhou510640,GuangdongProvince,China)Abstract:InordertoexplicitthecorrelationbetweenbiologicalcharacteristicandpathogenicityofIsariafumosorosea(=Paecilomycesfumosoroseus)toPlutellaxylostella,
5、fiveI.fumosoroseastrains,includingoriginalfatherstrainPFCFO,degeneratedfatherstrainPFCFD,singlesporeisolatesPFCFD58,PFCFD20,andPFCFD50,whichisolatedfromPFCFD,wereassayedonsporulation,growthrate,sporegerminaterate,extracellularproteaseandtheirpathogenicitytothe2ndinstarlarvaofP.xylostell
6、a.TheresultsshowedthatthegrowthrateofstrainPFCFOwasfastatinitialstageafterinoculatedtomedialbutsloweddownwiththetimeelapsed.ThestrainPFCFOappearedanobviouslyhighersporulation(2.7710conidial/mL)after20daysculture,sporegerminaterate(62.59%),extracellularproteaselevel(1.16)andcorrectedmortalit
7、y(97.74%)tothe2ndinstarlarvacomparedtootherstrains.However,amongthestrainsPFCFD,PFCFD20,PFCFD50andPFCFD58,therewerenosignificantdifferences7onthegrowthrateandsporulationgerminaterate.Sporulationofthesestrainswas1.0510-2.3471
