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1、6高脂飼料誘導的肥胖大鼠網(wǎng)膜素水平的實驗研究(400014重慶,重慶醫(yī)科大學附屬兒童醫(yī)院,兒童發(fā)育疾病研究省部共建教育部重點實驗室,兒科學重慶市重點實驗室,重慶市(兒童發(fā)育重大疾病診治與預防)國際科技合作基地)[摘要]目的人群調(diào)查發(fā)現(xiàn)肥胖人群網(wǎng)膜素水平較正常人群低,本研究擬檢測正常及肥胖大鼠血清網(wǎng)膜素水平及其基因表達情況。方法40只SD成年雄性大鼠隨機分為正常組(N=10)和高脂組(N=30),分別以普通飼料和高脂飼料喂養(yǎng)。六周后從高脂組選取體重增長最快的20只,從中隨機抽取10只繼續(xù)喂養(yǎng)高脂飼料,12周后兩組各剩9只,測量后處死并從心臟取血及留取
2、脂肪組織,采用全自動生化儀ADVIA2400測定血糖及血脂、ELISA檢測血清胰島素及網(wǎng)膜素、RT-PCR檢測網(wǎng)膜素mRNA表達水平。結(jié)果(1)高脂飼料喂養(yǎng)12周后,高脂組大鼠的體重,體重增加值及肥胖指數(shù)均高于正常組(分別為591.71gvs525.98g,286.59gvs211.89g,0.049vs0.027,P均<0.05);(2)高脂組低密度脂蛋白(0.29mmol/lvs0.20mmol/l)及胰島素水平(0.85ng/mlvs0.67ng/ml)高于正常組(P<0.05);(3)高脂組血清網(wǎng)膜素水平(99.68ng/mlvs56.98
3、ng/ml)及網(wǎng)膜脂肪組織網(wǎng)膜素mRNA表達水平(1.25vs1.06)均高于正常組(P均<0.05)。結(jié)論首次發(fā)現(xiàn)肥胖大鼠其血清網(wǎng)膜素水平及網(wǎng)膜脂肪組織中網(wǎng)膜素mRNA表達水平較正常大鼠顯著增高,與人群調(diào)查結(jié)果不一致。[關鍵詞]網(wǎng)膜素;網(wǎng)膜素mRNA表達;大鼠[中圖法分類號]R151.4;R-332;R723.14;R363.1[文獻標識碼]AIncreasedserumomentinanditsmRNAexpressioninhigh-fatdietinducedobeseratsLvPanpan,QuPing,BiYang,ChenJie,L
4、iuYouxue(Children’sHospitalofChongqingMedicalUniversity,Chongqing400014,China.MinistryofEducationKeyLaboratoryofChildDevelopmentandDisorders.KeyLaboratoryofPediatricsinChongqing,CSTC2009CA5002.ChongqingInternationalScienceandTechnologyCooperationCenterforChildDevelopmentandDis
5、orders)[Abstract]ObjectiveObesepopulationshavesignificantlylowerplasmaomentinlevelsthanleanones,ThepresentstudywastoinvestigatetheserumomentinlevelanditsmRNAexpressioninomentaladiposetissueinnormalandhighfatdiet-inducedobeserats.MethodsMaleadultSprague-Dawleyratswererandomlydi
6、videdinto2groups:10ratsincontrolgroupfedwithnormaldiet,30ratsinexperimentalgroupwithhighfatdiet(HFD).After6weeks,10ratswererandomlychosenfromthetop20ratswiththehighestweightgain.Theexperimentalrats(n=10)werefedwithHFDforanother6weeks.Atendofstudy,bloodsamplesweretakenfromheart
7、forthemeasurementofserumlipids,glucose,insulinandomentin.AdiposetissuesamplesweretakenfordeterminationofthemRNAexpressionofomentinbyRT-PCR.Results(1)After12weeksofhighfatdietfeeding,bodyweight,weightgainandtheobesityindexweresignificantlyhigherinHFDgroupratsthanthoseincontrolg
8、roup(525.98gvs591.71g,211.89gvs286.59g,0.027vs0.049,respectiv