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1、Ramantagimagingtechnology??Ramanspectroscopycanbeusedtodetectsmallmolecules?bydetectingtheirmolecularvibrations.However,?forobservationincells,Ramanscatteringfromsmall?moleculesareoftenoverlappedwiththosefromendogenous?molecules,andcannoteasilybeextracted.?Toovercome?th
2、isproblem,theso-calledRamantagtechnique?hasbeendevelopedtoseparatethetargetmoleculesfrom?others.??Ramantagimagingtakesadvantageof?thesilentregionintheRamanspectraofcells.Thesilentregionexistsbetween1800and2800cm_1wheremostbiologicalmoleculesdonotproduceRamanscatteringsi
3、gnals.Fortunately,functionalgroups,suchasalkyne,azide,deuterium,andnitrile,showcharacteristicRamanbandsinthesilentregion.Thus,thesesubstituentscanbeusedastagsforobservationofsmallmoleculesusingRamanscattering.Ref:?Palonpon,A.F.,etal.,2013.
