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1、876中華麻醉學雜志2014年々月第34卷第7期ChinJAnesthesiol，July2014，Vo1．34，No．7．·重癥醫(yī)學·苦碟子對力竭運動致大鼠心肌損傷時線粒體轉錄因子A表達的影響韓霜何錕趙亞冬容俊芳【摘要】目的評價苦碟子對力竭運動致大鼠心肌損傷時線粒體轉錄因子A(mtTFA)表達的影響。方法健康雄性Wistar大鼠4O只，體重200～220g，采用隨機數(shù)字表法分為對照組(c組，=8)、力竭運動組(Es組，n=16)和苦碟子組(Is組，n=16)。采用游泳力竭法建立力竭運動致大鼠心肌損傷模型。Is組于腹腔注射苦碟子注射液20g／kg后行力竭運動。Es組
2、及Is組于力竭后6和24h時分別隨機取8只大鼠，采集下腔靜脈血樣后摘取心臟，采用ELISA法檢測血清eTnI濃度，采用Westernblot法檢測心肌mtTFA蛋白表達。結果與c組比較，Es組及Is組力竭后6和24h時血清cTnI濃度升高，心肌mtTFA蛋白表達下調(P<0．05)；與Es組比較，Is組力竭后6和24h時血清cTnI濃度降低，心肌mtTFA蛋白表達上調(P<0．05)。結論苦碟子可通過上調心肌mtTFA蛋白表達減輕力竭運動致大鼠心肌損傷?！娟P鍵詞】菊科；運動過度；心肌疾??；線粒體；轉錄因子Effectofixerissonchifoliaonmito
3、chondriaitranscriptionfactorAexpressionfollowingmyocardialinjuryinducedbyexhaustingexerciseinratsHartShuang，HeKun，Zhaod0，舶Junfang．DepartmentofAnesthesiology，HebeiGeneralHospital，Shijiazhuang，050000，HebeiProvince，ChinaCorrespondingauthor：RongJunfang，Email：rjf13291@163．con【Abstract】Objec
4、tiveToevaluatetheefectofixerissonchifoliaonmitochondrialtranscriptionfactorA(mtTFA)expressionfollowingmyocardialinjuryinducedbyexhaustingexerciseinrats．MethodsFortymaleWistarrats，weighing200—220g，wererandomlydividedinto3groupsusingarandomnumbertable：controlgroup(groupC，n=8)，exhaustinge
5、xercisegroup(groupES，n=16)andixerissonchifoliagroup(groupIS，n=16)．Themodelofmyocardialinjurywasestablishedbyexhaustingswimming．InISgroup，theratsweresubjectedtoexhaustingswimmingafterintraperitonealixerissonchifolia20g／kg．InESandISgroups，8ratswerechosenat6and24hafterexhaustion，andbloods
6、amplesweretakenfromtheinferiorvenacavafordeterminationofsenlmcardiactroponinI(cTnI)concentrationsbyELISA．TheanimalsweresacrificedafterbloodsamplingandmyocardialspecimenswereobtainedformicroscopicexaminationandfordeterminationofmtTFAexpressionbyWesternblot．ResultsComparedwithgroupC，thec
7、oncentrationofselumcTn1wassignificantlyincreased，andtheexpressionofmtTFAinmyocardialtissueswasdown—regulatedat6and24hafterexhaustioninESand：ISgroups．ComparedwithgroupES，theconcentrationofserumcTn1wassignificantlydecreased，andtheexpressionofmtTFAinmyocardialtissueswasup-regulatedat6an
