高山紅景天立枯病菌菌絲融合群鑒定及藥劑敏感性-論文.pdf

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1、第42卷第1期東北林業(yè)大學學報Vol_42No．12014年1月J0URNALOFNORTHEASTFORESTRYUNIVERSITYJan．2014·--J同口山紅景天立枯病菌菌絲融合群鑒定及藥劑敏感性)白慶榮謝昀燁姜旭宇孫慧穎高潔(吉林農(nóng)業(yè)大學，長春，130118)摘要采用組織分離法從吉林省臨江市、安圖縣罹病高山紅景天植株分別獲得4、2個致病菌株。病菌的形態(tài)學特征、培養(yǎng)性狀、細胞核染色、茵絲融合及rDNA—ITS區(qū)序列測定結果表明：6個菌株均為茄立枯絲核菌(Rhi—zoctoniasolaniKfihn)，屬多核絲

2、核茵；臨江分離物Jl～J4為AG一4一HG1I菌絲融合群，安圖分離物J5一J6為AG—l—IB茵絲融合群。l9種殺菌劑對2個菌絲融合群的敏感性測定結果表明：2個茵絲融合群對蠟質(zhì)芽孢桿菌、枯草芽孢桿菌、井岡·蠟芽茵的敏感性最好(EC<0．005me,／L)；對多粘類芽孢桿菌和枯草芽孢桿菌(強爾)的敏感性次之(Ec<1．0ms／L)；對木霉菌和寡雄腐霉的敏感性略低(1．0ms／L

3、百茵清和咪鮮胺的敏感性略低(1．0ms／L

4、oJie(JilinAgriculturalUniversity，Changchun130118，P．R．China)／／JournalofNortheastForestryUniversity．-2014，42(1)．一107～l11Sixisolateswithpathogenicitywereobtainedfromthestemsofdiseasedplantswithtissueisolationmethod(fouriso—latesfromLinjiangCityandtwoisolatesfromAntu

5、County)．Theexperimentwasconductedtoinvestigatetheircharacteris—ticsofmorphologyandcultivation．RhizoctoniasolaniKtihnisthepathogenofthedisease．Anastomosisgroupsaredeter-minedtobeAG·—1—-IBsubgroupinAntuandAG-4-HGlIsubgroupinLinjiangbypairingisolateswithtestertrains

6、andse--quenceanalysisofrDNA—ITSregion．Byfungicidessusceptibilitytest，twoanastomosisgroupsaremostsensitivetoBacillusceFelhS，B．subtil~(10“／g)，Validamycin·B．cereus(EC50

7、igandrum(1．0ms／L

8、itivetocelest，hymexazolandhymexazolthanAG-4一HG11．KeywordsRhizoctoniasolanidampingof；Pathogenidentification；Anastomosisgroup；Susceptibility高山紅景天(Rhodiolasachali