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1、Gene520(2013)189–193ContentslistsavailableatSciVerseScienceDirectGenejournalhomepage:www.elsevier.com/locate/geneShortCommunicationAnalysisofgeneticvariabilityandpopulationstructureoftheendemicmedicinalLimoniumsinenseusingmolecularmarkersab,?,YuYanqiubaaDingGe,ZhangDaizhen,ZhaoLingling,ZhangBeibeia
2、ChemicalandBiologicalEngineeringCollege,YanchengInstituteofTechnology,Jiangsu,Yancheng224003,ChinabJiangsuProvincialKeyLaboratoryofCoastalWetlandBioresourcesandEnvironmentalProtection,YanchengNormalUniversity,Jiangsu,Yancheng224051,ChinaarticleinfoabstractArticlehistory:Limoniumsinenseisanendemicme
3、dicinalherbusedtotreatfever,hemorrhageandotherdisorders.IntheAccepted7March2013presentstudy,populationgeneticdiversitywaselucidatedusingrandomampli?edpolymorphicDNAAvailableonline16March2013(RAPD),intersimplesequencerepeat(ISSR)andampli?edfragmentlengthpolymorphism(AFLP)primers.Percentageofpolymorp
4、hicbands,Nei'sgenediversityandShannon'sinformationindexrevealedahighKeywords:levelofgeneticdiversityatspecieslevel.Theanalysisofmolecularvariancerevealedthat69.88%(RAPD),Limoniumsinense71.19%(ISSR)and70.97%(AFLP)ofvariabilitywerepartitionedamongindividualswithinpopulations,RAPDISSRwhichindicatedthe
5、coherenttrendbyGst(0.3849/0.3577/0.3670).Gene?ownumber(Nm)was0.581/AFLP0.618/0.612,whichindicatedthattherewasalimitedgeneexchangebetweenpopulations.TheUPGMAclus-Geneticvariabilityteringresultsshowedthatthegeneticdistancehadnosigni?cantcorrelationwithgeographicdistance.TheseConservationresultsindica
6、tethatthesemarkerswerereliabletoolsforthedifferentiationanddeterminationofthegeneticdiversityamongthepopulationsofL.sinenseandtheconservationofexistingnaturalpopulationisnecessary.?2013ElsevierB.V.Allrightsreserved.1.Introduction(AFLP)isaDNA?ngerprintingtechniquethatapproachestheidealmarkersystemfo
7、rresolvinggeneticdiversityamongindividuals,popu-Limoniumsinense,aperennialherbofthefamilyPlumbaginaceae,lationsandspecies(Jianetal.,2006;MuellerandWolfenbarger,1999).iswidelydispersedinsalineregionsthrougho
