資源描述：
《genetic stability analysis of diapause-induced》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫(kù)。
1、DOI:10.1111/j.1570-7458.2010.00978.xGeneticstabilityanalysisofdiapause-inducedmultivoltinesilkwormBombyxmorigermplasmusingintersimplesequencerepeatmarkersR.Saravanakumar,K.M.Ponnuvel*&S.M.H.QadriBiotechnologyLaboratory,CentralSericulturalGermplasmResourcesCentre,PBNo44,ThallyRoad,Hosur635
2、109,TamilNadu,IndiaAccepted:20January2010Keywords:Lepidoptera,Bombycidae,diapauseinduction,molecularmarkers,polymerasechainreaction,primers,phylogeneticrelationship,PCR,ISSR-PCRAbstractConservationofsilkworm[BombyxmoriL.(Lepidoptera:Bombycidae)]germplasmaidsinpreven-tionofgeneticerosionan
3、draceextinction.Multivoltinesilkwormgermplasmmaintenanceinvolvesrearing?vegenerationsperyearandthisrequiresexpenditure,manpower,andinfrastructure,inadditiontofrequentexposureofthesilkwormstodiseases,pests,andpredators.Tominimizetheseproblems,analternativeprotocolwasdevelopedbywayofinducti
4、onofeggdiapauseinthemultivol-tinesilkwormsthroughrearingofthefourthand?fthinstarsatlowtemperature(18–20°C)underregulatedphotoperiod(L6:D18).Thismethodallowedinductionofeggdiapauseintheselectedracesatlevelsrangingfrom26to94%andthediapause-inducedeggsweremaintainedundercoldpreser-vationcond
5、itions.Thestabilityofgenotypiccharacterswascon?rmedinthediapause-inducedsilkwormbatchesaftertheseventhgeneration,bycomparisonwithcontrolbatchesofsilkwormusingintersimplesequencerepeat-polymerasechainreaction(ISSR-PCR).AnalysisofNei’sgeneticdis-tanceforthedifferentracesindicatednosigni?can
6、tvariationbetweencontrolanddiapause-inducedraces.Thisrevealsthatalltheselectedracesmaintainedgeneticstabilityevenaftertheseventhgenera-tionatthephenotypicandmolecularlevel.Hence,itcanbeconcludedthatinductionofeggdiapauseisanappropriatealternativemethodtopreservemultivoltineracesforlongerp
7、eriodsoftime.tionofsilkwormgermplasmresourcesrepresentingracesIntroductionofvariousgeographicaloriginsthatalsorevealdiverseBombyxmoriL.(Lepidoptera:Bombycidae)isadomesti-morphological,reproductive,biochemical,growth,andcatedsilkwormandinviewofitslonghistoryofdomesti
