genetic diversity of natural miscanthus sinensis populations

genetic diversity of natural miscanthus sinensis populations

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時間:2018-02-10

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1、BiochemicalSystematicsandEcology48(2013)248–256ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversityofnaturalMiscanthussinensispopulationsinChinarevealedbyISSRmarkers111QiXiangZhang,Y

2、iKeShen,RuoXuanShao,JiaFang,YongQingHe,JunXiaRen,BingSongZheng*,GuoJuanChen*NurturingStationfortheStateKeyLaboratoryofSubtropicalSilviculture,ZhejiangA&FUniversity,Linan311300,ChinaarticleinfoabstractArticlehistory:MiscanthussinensisisatypicalperennialC4grassinAsia.Intheprese

3、ntstudy,ISSRReceived5September2012markerswereusedtoevaluatethegeneticvariationwithinandamonggrasspopulationsAccepted24December2012thatweresampledfromtheZhejiangandGuangdongProvincesofChina.BasedonnineAvailableonline12February2013ISSRprimers,206clearandreproducibleDNAfragments

4、weregenerated.Relativelylowlevelsofgeneticdiversityweredeterminedamongthepopulations.Thecoef?cientofKeywords:geneticdifferentiationamongthepopulationswas0.52,whichindicatesthat52.3%oftheMiscanthussinensistotalmolecularvarianceexistsamongthepopulations.Suchahighlevelofdivergen

5、ceGeneticdiversitypresentamongthepopulationsmightbecausedbythecomplextopographyandvariableDifferentiationISSRclimaticconditionspresentinthetwoprovinces.AMOVArevealedthatthemajorityofthegeneticvariationwaswithinthepopulations(50.6%).Theapplicationofanovelmethod,whichcombinesge

6、ographicalcoordinatesandgeneticdifferentiationtodetectbarriersforgene?ow,allowedustoidentifytwozonesofloweredgene?ow.ó2013ElsevierLtd.Allrightsreserved.1.IntroductionEnergycrisisandenvironmentaldegradationhaveledtothesearchforalarge-scalesubstitutionoffossilfuel(Ceotto,2008;B

7、enbiandBrar,2009).Manybotanistshavebeentryingto?ndplant-basedbiofuelsinordertoreducetheuseoffossilfuel.Amongthem,Miscanthusisoneofthemostimportantbiofuelplantsthathastheadvantagesoffastgrowth,lowinput,andhighphotosynthesis(VisserandPignatelli,2001;Clifton-Brownetal.,2004;Hast

8、ingsetal.,2008),andiswidelyusedinEuropeasabioenergycrop.Miscanthusis

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