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1、BiochemicalSystematicsandEcology39(2011)139–144ContentslistsavailableatScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversitywithinandamongthewildpopulationsofMurrayakoenigii(L.)Spreng.,asrevea
2、ledbyISSRanalysisSushmaVerma,T.S.Rana*ConservationBiologyandMolecularTaxonomyLaboratory,NationalBotanicalResearchInstitute(CouncilofScienti?candIndustrialResearch),RanaPratapMarg,Lucknow226001,IndiaarticleinfoabstractArticlehistory:Murrayakoenigii(L.)Sp
3、reng.,commonlyknownascurryleafplant,isfoundinthedifferentReceived10August2010hillyregionsofIndia.Inthepresentstudy,?fty-nineaccessionsrepresentingeightwildAccepted29January2011populationsofM.koenigiiwereanalyzedusingthirteenISSRprimers.Atotalof152bandsA
4、vailableonline22February2011wereampli?ed,outofwhich,136werepolymorphiccorrespondingto89.47%polymorphismacrosstheaccessions.ThepairwisepopulationgeneticdistanceswerecalculatedforalltheKeywords:populationsthatvariedfrom0.05to0.13betweenthepopulationsofM.k
5、oenigii.AMOVAandGeneticdiversityNei’sgeneticdiversityanalysisrevealedhighergeneticvariationswithinpopulationsthanPopulationstructureamongthepopulations.TheclusteringofpopulationsinthedendrogramwasnotinWildMurrayakoenigiiISSRcongruencewithgeographicalaf?
6、liations.TheresultsindicatethattheISSRmethodissuf?cientlyinformativeandpowerfultoestimatethegeneticdiversityinM.koenigiipop-ulations.AsM.koenigiiisanimportantwildplantgeneticresource,therefore,informationongeneticvariabilitymightbeapotentialsourceasbree
7、dingmaterialfordevelopmentofcommerciallyvaluabletraitsinM.koenigiiplants.ó2011ElsevierLtd.Allrightsreserved.1.IntroductionThegenusMurrayaKoenigexL.,(Rutaceae)comprisesofabout11speciesofshrubsandsmalltreesdistributedintropicalandsub-tropicalregionsofSriL
8、anka,India,SouthChinatoSouth-EastAsia,Malaysia,NewGuinea,North-EastAustraliaandNewCaledonia(SwingleandReece,1967).InIndiathegenusisrepresentedbytwospecies,Murrayakoenigii(L.)Spreng.,andMurrayapaniculata(L.)Jack.,having(2n)18chrom
