genetic relationship between chinese wild vitis species and american

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1、BiochemicalSystematicsandEcology46(2013)120–126ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticrelationshipbetweenChinesewildVitisspeciesandAmericanandEuropeanCultivarsbasedonISSRmarkersa,ba,b,*ZhaobinJing,Xipi

2、ngWangaCollegeofHorticulture,NorthwestA&FUniversity,KeyLaboratoryofHorticulturalPlantBiologyandGermplasmInnovationinNorthwestChina,MinistryofAgriculture,Yangling,Shaanxi712100,ChinabStateKeyLaboratoryofCropStressBiologyinAridAreas,NorthwestA&FUniversity,Yangling,Shaanxi712100,Chinaarticleinfoabstra

3、ctArticlehistory:Inter-simplesequencerepeat(ISSR)markerswereemployedtodetectthegeneticdiversityReceived29January2012among70grapeaccessionsincluding52clonesof17Chinesewildgrapespecies,sevenAccepted30August2012interspeci?chybrids,10VitisviniferaL.cultivars,andonestrainofVitisripariaL.AtotalofAvailabl

4、eonline16October2012119polymorphicbandswithanaverageof11.9perprimerwereobserved.Theunweightedpair-groupmethod(UPGMA)analysisindicatedthatthe70clonesoraccessionshadKeywords:asimilarityrangefrom0.08to0.93,indicatingthatabundantdiversitiesexistamongtheseChinesewildVitisspeciesaccessions.Basedoncluster

5、analysisandprincipalcoordinateanalysis,allaccessionscouldVitisbedividedintotwomajorgroups,theChinesewildgrapegroup,andtheAmericanandGeneticdiversityISSREuropeancultivargroup.ThelargestdistancewasfoundamongV.ripariaMichX,Vitispiasezkii,V.viniferaL.interspeci?chybrid(VitisbiniferaV.labrusca)andthewi

6、ldgrapesnativetoChina.ó2012ElsevierLtd.Allrightsreserved.1.IntroductionGrape(VitisviniferaL.)isoneoftheoldestandmostimportantfruitcropsintheworld.TheVitisgenuscontainsmorethan70species,withcentersoforigininsouthEurope,AsiaMinor,EastAsia,andNorthandCentralAmerica(Wanetal.,2008).Chinaisoneofthemajorg

7、ermplasmcentersofVitisspeciesandmorethan35VitisspeciesoriginateinChina(Wanetal.,2008).ChinesewildVitishasmanyusefulcharacteristics,including(i)highqualitywinemakingattributes,suchashighsugarcontentandmodera