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1、Amoebaandflagella阿米巴和鞭毛蟲(chóng).Entamoebahistolytica(E.histolytica)溶組織內(nèi)阿米巴.Giardialamblia(G.lamblia)蘭氏賈第鞭毛蟲(chóng).Trichomonasvaginalis(T.vaginalis)陰道毛滴毛蟲(chóng).Leishmaniadonovani(L.donovani)杜氏利什曼原蟲(chóng)PurposeandRequirements.MastermorphologicalfeaturesoftrophozoiteandcystofEntamoebahist
2、olyticaandGiardialamblia;Mastermovementcharacteristicsoftrophozoites;.MastermorphologicalfeaturesandmovementcharacteristicsofTrichomonasvaginalis;.FamiliarwithdiffertiationsofE.histolyticacystandothernon-pathogenicamoebaecyst;.Familiarwithiodinestainforcyst;.Unde
3、rstandingBalantidiumcoli;.UnderstandingmorphologicalfeaturesofamastigoteofLeishmaniadonovaniandsandfly..1.獨(dú)立觀察玻片標(biāo)本No.原-1至No.原-4:2.示教內(nèi)容學(xué)習(xí):1)圖片及視頻示教:2)鏡下示教標(biāo)本:3.完成實(shí)驗(yàn)操作:①直接涂片法觀察活動(dòng)的阿米巴滋養(yǎng)體;②直接涂片法觀察活動(dòng)的陰道毛滴蟲(chóng);③碘染色法觀察包囊4.完成實(shí)驗(yàn)報(bào)告:本次課學(xué)習(xí)任務(wù)1.ObservationofglassslideNo.原-1至No.原-4
4、拿到玻片標(biāo)本后請(qǐng)馬上檢查核對(duì)是否有漏、重復(fù)或破損,有問(wèn)題的請(qǐng)馬上舉手報(bào)告老師!No.原-1:溶組織內(nèi)阿米巴包囊、結(jié)腸內(nèi)阿米巴包囊、微小內(nèi)蜓阿米巴包囊、布氏嗜碘阿米巴包囊CystofE.histolytica,E.coli,E.nana,I.buetschliiNo.原-2:藍(lán)氏賈第鞭毛蟲(chóng)滋養(yǎng)體G.lambliatrophozoite(僅雙號(hào)同學(xué)有,2個(gè)同學(xué)共用1張)No.原-3:藍(lán)氏賈第鞭毛蟲(chóng)包囊G.lambliacystNo.原-4:陰道毛滴蟲(chóng)滋養(yǎng)體T.vaginalistrophozoite注意:①在觀察玻片標(biāo)本前,
5、先將顯微鏡光線調(diào)到最強(qiáng)?、诒敬握n只使用10倍鏡和油鏡;No.原-4:陰道毛滴蟲(chóng)滋養(yǎng)體T.vaginalistrophozoiteAxostyle軸柱4anteriorflagellum4根前鞭毛LateralflagellumandUndulatingmembrane波動(dòng)膜Nucleus1.ObservationofglassslideNo.原-1至No.原-4先10倍鏡下調(diào)出焦距平面,然后轉(zhuǎn)油鏡下觀察細(xì)微結(jié)構(gòu)要求拍照姬姆薩染色法(Giemsastainingmethod)No.原-3:藍(lán)氏賈第鞭毛蟲(chóng)包囊G.lambli
6、acystSteinedwithiron-hematoxylinCystwall囊壁Nucleus核Axostyle軸柱Medianbody中體.Cystwall:.Axostyle:.Medianbody:.Nucleus:1.ObservationofglassslideNo.原-1至No.原-4先10倍鏡下調(diào)出焦距平面,然后轉(zhuǎn)油鏡下觀察細(xì)微結(jié)構(gòu)要求拍照No.原-2:藍(lán)氏賈第鞭毛蟲(chóng)滋養(yǎng)體G.lambliatrophozoiteAnteriorflagellum前鞭毛Ventralflagellum腹鞭毛Posteri
7、orflagellum后鞭毛Caudalflagellum尾鞭毛Adhesivedisc吸盤(pán)NucleusMedianbody中體Axostyle軸柱1.ObservationofglassslideNo.原-1至No.原-4先10倍鏡下調(diào)出焦距平面,然后轉(zhuǎn)油鏡下觀察細(xì)微結(jié)構(gòu)要求拍照姬姆薩染色法(Giemsastainingmethod)No.原-2:藍(lán)氏賈第鞭毛蟲(chóng)滋養(yǎng)體G.lambliatrophozoiteSteinedwithiron-hematoxylinAdhesivediscandnucleus吸盤(pán)和核Med
8、ianbody中體Axostyle軸柱1.ObservationofglassslideNo.原-1至No.原-4先10倍鏡下調(diào)出焦距平面,然后轉(zhuǎn)油鏡下觀察細(xì)微結(jié)構(gòu)要求拍照No.原-1:溶組織內(nèi)阿米巴包囊、結(jié)腸內(nèi)阿米巴包囊、微小內(nèi)蜓阿米巴包囊、布氏嗜碘阿米巴包囊CystofE.histolytica,E.coli,E