資源描述：
《基因敲除模式小鼠的飼養(yǎng)與交配》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、基因敲除模式小鼠的飼養(yǎng)與交配Whatareweusuallyusing?Painforlaboratorymicerevealedin'human'facialexpressionsGeneticallyEngineeredMouseModelsResource?GiftTransgenicKnockout?MadebyyourownKnockin?MadebyBiocytogenGeneralprinciplesforbreedingFundamentalsofMouseReproductionSexualmaturity:6~8weeksReproductivelifespan:7
2、~8monthsFertilityGestation:18~21daysGenerationtime:12weeksLittersize:2~12pups/litterWeaningage:18~28daysBreedingPerformanceFactorsHybridvigor---more,larger,andhealthierlittersthaninbredstrains.Strain-specificbehaviors---AggressivebehaviorsorpoormothersMutationsandtransgeneeffects---Embryonicleth
3、al,orinfertilityorreducedfertilityOptimizingBreedingPerformanceReplacebreedersregularlyReplacenon-productivebreeders~60daysMatemiceearlyUseexperiencedmalesKeepaccuratebreedingrecordsOptimizingBreedingPerformanceLitterfosteringDeterminingpregnancyMatingnumerousfemalessimultaneouslyManagingSmallCo
4、loniesMaintainaminimumofsixbreedingpairsKeeptheagerangeofyourbreedersbetweentwoandeightmonthsoldRetaintwogenerationsofastrain,anddonoteliminateoneuntilthenextoneisproducingMonitorbreedingperformancecloselyConsidercryopreservingastrainBreedingSchemesChoosingaBreedingSchemeWhatgenotypesdoIneed?mos
5、tefficientwaytoproducethem?Willappropriatecontrolsbeavailable?geneticbackgroundHowaregenotypesidentified?phenotype?molecularassay(e.g.PCR)?WhataboutLinkage?X-linkedormultiplegenes?Reproductiveissues(sterility)Phenotypeissues(lethality)SimpleBreedingSchemes+/-+/+HeterozygousWildtype+/-+/++/-Heter
6、ozygousWildtype50%50%wildtypeKnockoutSimpleBreedingSchemes+/-+/-HeterozygousHeterozygous-/-+/-+/+HomozygousHeterozygousWildtype25%50%25%SimpleBreedingSchemes+/--/-HeterozygousHomozygous+/--/-HeterozygousHomozygous50%50%BackcrossingbreedingN1N#=backcrossgenerationnumberF#=brotherXsistergeneration
7、numberComplexBreedingSchemesTransgenicmiceTransgenicmice?Foundermice:transgenicmicethatdevelopfromtheinjectedeggs?Transgeniclines:derivedfromthesefoundersBreedingSchemesfortransgenicmiceGenotypingstrategyTg-FTg/++/+Heterozyg
