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1、華南農(nóng)業(yè)大學(xué)學(xué)報2015,36(3):7882http:∥xuebao.scau.edu.cnJournalofSouthChinaAgriculturalUniversitydoi:10.7671/j.issn.1001411X.2015.03.014劉云華,吳毅歆,楊紹聰,等.洋蔥伯克霍爾德溶磷菌的篩選和溶磷培養(yǎng)條件優(yōu)化[J].華南農(nóng)業(yè)大學(xué)學(xué)報,2015,36(3):7882.洋蔥伯克霍爾德溶磷菌的篩選和溶磷培養(yǎng)條件優(yōu)化12,3411,2劉云華,吳毅歆,楊紹聰,何鵬飛,何月秋(1云南農(nóng)業(yè)大學(xué)農(nóng)業(yè)生物多樣性應(yīng)用技術(shù)國家工程研究中心,云南昆
2、明650201;2云南農(nóng)業(yè)大學(xué)農(nóng)學(xué)與生物技術(shù)學(xué)院,云南昆明650201;3微生物菌種篩選與應(yīng)用國家地方聯(lián)合工程研究中心,云南昆明650217;4玉溪市農(nóng)業(yè)科學(xué)院,云南玉溪653100)摘要:【目的】從磷礦區(qū)植物根圍土中,篩選出具有較高溶磷能力的菌株及優(yōu)化溶磷培養(yǎng)條件.【方法】通過稀釋#板法分離、篩選菌株,并進(jìn)行全細(xì)胞脂肪酸分析和16SrDNA測序鑒定;正交試驗(yàn)優(yōu)化溶磷培養(yǎng)條件.【結(jié)果和結(jié)論】-1篩選出22株具有溶磷能力的菌株,其中菌株YN2014102的溶磷能力最強(qiáng),在10g·L的磷礦粉培養(yǎng)基中能釋放-1244.75mg·L水溶性磷.經(jīng)全細(xì)胞脂肪
3、酸分析和16SrDNA測序,該菌株被鑒定為洋蔥伯克霍爾德菌.正交試驗(yàn)-1-1的結(jié)果表明,在質(zhì)量濃度10g·L的磷礦粉、28℃、搖床轉(zhuǎn)速為180r·min的條件下培養(yǎng)5d,溶磷效果最好,達(dá)-1277.08mg·L.關(guān)鍵詞:溶磷細(xì)菌;磷礦粉;氣相色譜分析;全細(xì)胞脂肪酸;16SrDNA中圖分類號:S154.3文獻(xiàn)標(biāo)志碼:A文章編號:1001411X(2015)03007805ScreeningofphosphorussolubilizingstrainBurkholderiacenocepaciaandoptimizingofphosphate
4、dissolvingculturecondition12,3411,2LIUYunhua,WUYixin,YANGShaocong,HEPengfei,HEYueqiu(1NationalEngineeringCenterforAppliedTechniquesofAgriculturalBiodiversity,YunnanAgriculturalUniversity,Kunming650201,China;2FacultyofAgronomyandBiotechnology,YunnanAgriculturalUniversity,Kunm
5、ing650201,China;3NationalandLocalJointEngineeringResearchCenterforScreeningandApplicationofMicrobialStrains,Kunming650217,China;4YuxiAcademyofAgriculturalSciences,Yuxi653100,China)Abstract:【Objective】Toisolateandscreenphosphorussolubilizingstrainsfromtherhizosphericsoilinpho
6、sphateorezoneandtooptimizeitsphosphorussolubilizingcondition.【Method】Dilutionandplatingwereappliedtoscreenandpurifyphosphorussolubilizingstrains.Thewholecellfattyacidwasanalyzedbygaschromatographyand16SrDNAsequencewasidentified.Theorthogonaldesigntestwasusedinoptimizingph
7、osphorussolubilizingcondition.【Resultandconclusion】Total22phosphorussolubilizingstrainswereobtainedbythetraditionalmethodologies.YN2014102strainshowedastrongabilitytodissolvephos-1-1phate,whichcouldget244.75mg·Lavailablephosphorusfromthe10g·Lphosphaterockmedium.YN2014102st
8、rainwasidentifiedasBurkholderiacenocepaciabasedonitsgaschromatograph