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1、去瓶歷雖翻麵値兔醐麵傷姓名,2014年6月25日去抗原同種異體靜脈套接修復兔面神經(jīng)損傷【摘?!恳越?jīng)生物工程處理的靜脈導管為支架,應用兔為動物實驗對象,觀察該方式修復面神經(jīng)損傷的特點?!痉椒ā?4只新西蘭大白兔,每只實驗兔分別應用靜脈導管及自體神經(jīng)移植吻合修復面神經(jīng)損傷,手術后行大體觀察、生物機能實驗系統(tǒng)記錄祌經(jīng)動作電位、切片組織學檢查?!窘Y(jié)果】活體動物手術后觀察,靜脈導管側(cè)胡須運動、上唇口輪匝肌運動及其運動幅度比自體神經(jīng)組稍多;生物機能實驗系統(tǒng)記錄神經(jīng)動作電位,術后10周和15周時靜脈導管側(cè)和自體神經(jīng)側(cè)均可引出神經(jīng)動作電
2、位,靜脈導管側(cè)的神經(jīng)傳導速度平均值均高于CJ體神經(jīng)側(cè);術后10周,各兔再生神經(jīng)組織化學染色,靜脈導管側(cè)的再生冇髓神經(jīng)纖維分布密集,并見分裂成熟的面祌經(jīng)結(jié)構,自體祌經(jīng)側(cè)染色的祌經(jīng)纖維分布稍稀疏,再生的有髓神經(jīng)結(jié)構不完全成熟?!窘Y(jié)論】利用生物性天然靜脈導管,經(jīng)生物工程技術處理,克服了天然生物材料引起衍主的免疫排斥反應,比應用S體神經(jīng)移植更宥利于促進神經(jīng)再生和功能恢復。【關鍵詞】生物工程靜脈導管修復面祌經(jīng)損傷Abstract:【Objective】Therepairofthefacialnerveinjuryofrabbits
3、wasexploredbyusingthevenousconduits.【Methods】Thefacialnerveinjuryoffifty-fourmaleNewZealandrabbitsweretreatedbyvenousconduitsandautonervegrafts.Afteroperations,themacroscopicobservations,detectionsofnerveactionpotentialsandhistoIogica1evaluationsonrabbitswereperf
4、ormed.【Results】Macroscopicfindings:Thefrequencyandamplitudeofmovementsofvibrissaeandorbicularisorismuscleoftheupperlipwereimprovedalittleinthesidesofvenousconduitscomparedwiththatinthesidesofautografts.Electrophysiologicalexaminations:nerveactionpotentia1scouldbe
5、detectedinbothsidesofvenousconduitsandautograftsat10weeksand15weekspostoperatively,respectively.Themeanvelocityofnerveconductioninthesidesofvenousconduitswasgreaterthanthatinthesidesofautografts.Histologicexaminations:at10weekspostoperatively,inthesidesofvenousco
6、nduit,regeneratedmedullatednervefibersweredistributeddenselywithmaturefacia1nervestructure.Whileinthesidesofautografts,regeneratednervefibersweredistributedsparselywithimmaturemedullatednervestructure.【Conclusion】Thenaturalvenousconduitprocessedbybioengineeringte
7、chnologyaddressestheimmunologicrejectionofthehost,andofferedgreaterpromotioninregardtoregenerationandfunctionalrecoveryofnervescomparedwithautologousnervegraftKeywords:bioengineering;venousconduit;repair;facialnerveinjury面神經(jīng)損傷可并發(fā)于巖骨骨折,也可為橋腦、小腦腳腫瘤或顱底腫瘤手術引起[1-3]。而神
8、經(jīng)是而部肌肉主耍的運動神經(jīng),對維持而部在靜態(tài)、動態(tài)的勻稱和面部表情的產(chǎn)生其宥重要作用。面神經(jīng)損傷引起眼瞼閉合不全、面肌癱瘓、蔞縮,最終引起失明、面部畸形,嚴重影響面部美觀、功能及患者的n常生活及社會活動。面祌經(jīng)損傷后再生是一個復雜的過程,涉及生物、生理、化學等諸多因素,人們尚未對這一過程徹底解決。近兒十年來,隨著妞微