雷蘑液態(tài)發(fā)酵工藝響應(yīng)面法優(yōu)化研究

《雷蘑液態(tài)發(fā)酵工藝響應(yīng)面法優(yōu)化研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、ChineseAgriculturalScienceBulletinVol.22No.62006June·76·http://www.casb.org.cn食品科學(xué)雷蘑液態(tài)發(fā)酵工藝響應(yīng)面法優(yōu)化研究王永斌1,21,3,王允祥(1蚌埠學(xué)院食品科學(xué)與工程系,安徽蚌埠233000;2南京農(nóng)業(yè)大學(xué)食品科技學(xué)院,江蘇南京210095;3浙江林學(xué)院食品與藥學(xué)學(xué)院,浙江臨安311300;)摘要:在雷蘑(Clitocybegigantean)AS5.105液態(tài)發(fā)酵培養(yǎng)基優(yōu)化的基礎(chǔ)上,采用Plackett-Burm

2、an設(shè)計(jì)(Plackett-BurmanDesign,P-B)對(duì)影響雷蘑發(fā)酵胞外多糖的內(nèi)在和外在因素進(jìn)行了篩選,所選取的6個(gè)相關(guān)因素為:初始pH,培養(yǎng)溫度,發(fā)酵時(shí)間,裝液量,接種量,搖床轉(zhuǎn)速。在此基礎(chǔ)上,再采用響應(yīng)曲面法(ResponseSurfaceMethodology,RSM)對(duì)影響雷蘑發(fā)酵胞外多糖的關(guān)鍵影響因素培養(yǎng)溫度,發(fā)酵時(shí)間和裝液量的最佳水平范圍作了進(jìn)一步的研究與探討,通過對(duì)二次多項(xiàng)回歸方程求解得知,在上述自變量分別為培養(yǎng)溫度27.05℃、發(fā)酵時(shí)間6.92d和裝液量93.90ml時(shí),

3、胞外多糖產(chǎn)量的最大預(yù)測值為103.58mg/100ml。關(guān)鍵詞:雷蘑(Clitocybegigantean)AS5.105;胞外多糖;Plackett-Burman設(shè)計(jì);響應(yīng)曲面法(RSM);Box-Behnken設(shè)計(jì)中圖分類號(hào):Q920.6文獻(xiàn)標(biāo)識(shí)碼:AOptimizationofSubmergedFermentationConditionsforExtracellularPolysaccharidebyClitocybegiganteanUsingResponseSurfaceMethodo

4、logy1,21,3WangYongbin,WangYunxiang(1DepartmentofFoodScienceandEngineering,BengbuCollege,AnhuiBengbu233000;2CollegeofFoodScienceandTechnology,NanjingAgriculturalUniversity,NanjingJiangsu210095;3CollegeofFoodandPharmacy,ZhejiangForestryUniversity,Lin'a

5、nZhejiang311300)Abstract:OurpreviousworkhasindicatedthatcultivationconditionsforextracellularpolysaccharidebyClitocybegiganteanarestronglyaffectedbymanyinternalandexternalfactors.Inthisstudy,weuseaneffectivetwo-phasestatisticalapproachtoenhanceextrac

6、ellularpolysaccharideproduction.Inthefirstphase,Plackett-Burmandesignwasundertakentoevaluatetheeffectsofthesixfactors,includinginitialpH,culturetemperature,fermentationtime,volumeofmedium,amountofinoculumandrotatespeed.Byregressionanalysis,culturetem

7、perature,timeandamountofinoculumwerefoundtobeimportantforcultivationconditionsforextracellularpolysaccharidebyClitocybegigantean.Inthesecondphaseoftheoptimizationprocess,aresponsesurfacemethodology(RSM)wasusedtooptimizetheabovecriticalfactors,andtofi

8、ndouttheoptimalconcentrationlevelsandtherelationshipsbetweenthesefactors.Basedontheresultsofthefirstphase,athree-levelthree-factor(culturetemperature,fermentationtimeandvolumeofmedium)Box-Behnkendesignwasemployed.Bysolvingthequadraticregressionmodele