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1、ChineseAgriculturalScienceBulletinVol.22No.62006June·76·http://www.casb.org.cn食品科學雷蘑液態(tài)發(fā)酵工藝響應面法優(yōu)化研究王永斌1,21,3,王允祥(1蚌埠學院食品科學與工程系,安徽蚌埠233000;2南京農(nóng)業(yè)大學食品科技學院,江蘇南京210095;3浙江林學院食品與藥學學院,浙江臨安311300;)摘要:在雷蘑(Clitocybegigantean)AS5.105液態(tài)發(fā)酵培養(yǎng)基優(yōu)化的基礎上,采用Plackett-Burm
2、an設計(Plackett-BurmanDesign,P-B)對影響雷蘑發(fā)酵胞外多糖的內(nèi)在和外在因素進行了篩選,所選取的6個相關因素為:初始pH,培養(yǎng)溫度,發(fā)酵時間,裝液量,接種量,搖床轉速。在此基礎上,再采用響應曲面法(ResponseSurfaceMethodology,RSM)對影響雷蘑發(fā)酵胞外多糖的關鍵影響因素培養(yǎng)溫度,發(fā)酵時間和裝液量的最佳水平范圍作了進一步的研究與探討,通過對二次多項回歸方程求解得知,在上述自變量分別為培養(yǎng)溫度27.05℃、發(fā)酵時間6.92d和裝液量93.90ml時,
3、胞外多糖產(chǎn)量的最大預測值為103.58mg/100ml。關鍵詞:雷蘑(Clitocybegigantean)AS5.105;胞外多糖;Plackett-Burman設計;響應曲面法(RSM);Box-Behnken設計中圖分類號:Q920.6文獻標識碼:AOptimizationofSubmergedFermentationConditionsforExtracellularPolysaccharidebyClitocybegiganteanUsingResponseSurfaceMethodo
4、logy1,21,3WangYongbin,WangYunxiang(1DepartmentofFoodScienceandEngineering,BengbuCollege,AnhuiBengbu233000;2CollegeofFoodScienceandTechnology,NanjingAgriculturalUniversity,NanjingJiangsu210095;3CollegeofFoodandPharmacy,ZhejiangForestryUniversity,Lin'a
5、nZhejiang311300)Abstract:OurpreviousworkhasindicatedthatcultivationconditionsforextracellularpolysaccharidebyClitocybegiganteanarestronglyaffectedbymanyinternalandexternalfactors.Inthisstudy,weuseaneffectivetwo-phasestatisticalapproachtoenhanceextrac
6、ellularpolysaccharideproduction.Inthefirstphase,Plackett-Burmandesignwasundertakentoevaluatetheeffectsofthesixfactors,includinginitialpH,culturetemperature,fermentationtime,volumeofmedium,amountofinoculumandrotatespeed.Byregressionanalysis,culturetem
7、perature,timeandamountofinoculumwerefoundtobeimportantforcultivationconditionsforextracellularpolysaccharidebyClitocybegigantean.Inthesecondphaseoftheoptimizationprocess,aresponsesurfacemethodology(RSM)wasusedtooptimizetheabovecriticalfactors,andtofi
8、ndouttheoptimalconcentrationlevelsandtherelationshipsbetweenthesefactors.Basedontheresultsofthefirstphase,athree-levelthree-factor(culturetemperature,fermentationtimeandvolumeofmedium)Box-Behnkendesignwasemployed.Bysolvingthequadraticregressionmodele
