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1、...a,。20{)6565ngy2006,o。,5320中華消化雜志年月第2卷第期chilDiMvl26N不匕牢字高脂飲食非酒精性脂肪性肝病大鼠肝臟成脂相關(guān)基因表達(dá)增強(qiáng)范建高方繼偉陸元善田麗艷徐正婕[摘要]目的探討非酒精性脂肪性肝病(NAFLD)大鼠肝臟成脂相關(guān)基因mRNA表達(dá)的動(dòng)態(tài)變化。方法模型組SD大鼠給予高脂飲食飼養(yǎng),分批于實(shí)驗(yàn)第4、8、12、16、24周處死,同期設(shè)普通飲食。一RPC尺]C〔SREBPIc)及飼養(yǎng)大鼠作對(duì)照平分別檢測(cè)肝臟固醇調(diào)節(jié)元件結(jié)合蛋自其靶基因脂肪酸合成、、。.酶(FAS)脂聯(lián)素抵杭素mRNA表達(dá)結(jié)果模型組大鼠4周肝臟可見散在性肝
2、細(xì)胞脂肪變性8周為,。,單純性脂肪性肝炎12~24周從脂肪性肝炎進(jìn)展為脂肪性肝炎伴肝纖維化從實(shí)驗(yàn)第4周起模型組大鼠cl,.SREBP-l和llRNA5一62~25;肝臟FAS表達(dá)逐漸增強(qiáng)至24周時(shí)分別較對(duì)照組升高倍和倍模型組大鼠肝臟從第12周起出現(xiàn)脂聯(lián)素和抵抗素RNA,。m表達(dá)兩者表達(dá)量均隨造模時(shí)間延長而增強(qiáng)相關(guān)分析顯.,、、、、.、SRE13PI。FASRNA0808。4示脂聯(lián)素抵抗素m表達(dá)量均與肝脂肪變程度呈正相關(guān)(r值分別為83..、,.。_~、、、仁。592。577尸值均<。01)結(jié)論高脂飲食N八FlD大鼠肝臟SREBPlFAS脂聯(lián)素抵抗素等成脂基因表
3、達(dá)增強(qiáng),提示脂肪變性的肝細(xì)胞可能部分具有脂肪細(xì)胞的特征.即發(fā)生r成脂性改變?！娟P(guān)鍵詞】脂肪肝,;;;非酒精性固醇調(diào)節(jié)元件結(jié)合蛋白脂聯(lián)素抵抗素ove卜exPressionofadiPogenicgenesintheliverofratswithnonaleoholiefattyliverdiseaseindueedbyhigh-一,一一,.e:a、aoe:a二、an。zaenzerrazz:二。r,a,,gha;r、zfatditl幾八NJgFANGJiwLUYuhlCf.)F夕LShiFeoe‘、osza,Zaozon:。er、2夕pPz月PizJgUn1Sh
4、anghaz200080,Ch;,,aAbstractOeetiveToinvestigateexpress舊nofmessagcRNAofadipogenlegenesintherats[lbjvereveonnon一aeooealversease.eoseratsoeasesta1iwithdlpiglhlifttyldiMthdThSDrndlwl)lishbyee一natet.enteoaratsoresaereea,,anee.fdghighfdiThhrndlwifidt4thsth12thdZ在thwksrcspeet一vely.一Therats
5、reeeivednoraetswereaseontrolSeuanttat一ve一asusetoeteetteexres-mldimiqiRTPCRwddhp5ionosteroreguatoryeeent一nngproteinle一leanditstargetgeneattyaeidsyntase,flllmbidi(SREBP)fhadlpsinandresistinintheIiver.ResultsSottyhepatiesteatosisansimPlettyliverwereobseenthepdfarvdimodelgroupat4thandst
6、hweeks,respeetively.From12thweekto24thwcek,theliversgraduallyprogressed.romsteatoeattstorossoaretteeontrou,teexressono一eanfhpiifibiCdwihhlgrophpifSREBpldFAS呷ml又NAwasureuatenteveroerou,exres,onoeanereetenepgldihlifdlgpm嘆NApifSREBP-ldFASwhighdmo一an一.oesatteee,reseetve.osve1llexrbys6dZ
7、25fldh24thwkpilyPitiRNApes、ionofadiPslnandresistinin.teoelgrouPwereoeeurredatte12tee,annereasedwitthetimeAosltveeorreatona、hmdhhwkdihpiliw一,,,oundbetweenteexpressionoleFASasinresistinanepatiesteatosls(rfhmRNAfSREBPdipdh-.,.....·08080834,0592,0577,P<001)ConelusionsTheexPressionSREBP一
8、le,FAS,adipsinresis
