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1、菏澤醫(yī)學(xué)?？茖W(xué)校學(xué)報(bào)VOL．22NO．4第22卷第4期2010年JOURNALOFHEZEMEDICALCOLLEGE2010doi:10．3969/j．issn．1008－4118．2010．04．02大鼠實(shí)驗(yàn)性牙移動(dòng)中Integrin－αm的表達(dá)變化喬婷①，陳杰②(青島大學(xué)醫(yī)學(xué)院，山東青島266071;青島大學(xué)醫(yī)學(xué)院附院，山東青島266071)摘要目的探討大鼠牙齒移動(dòng)后Integrin－αm在三叉神經(jīng)節(jié)、腦干三叉神經(jīng)脊束核、大腦皮層的表達(dá)情況，借以了解正畸治療初期慢性痛的神經(jīng)傳導(dǎo)通路及Integrin－αm在慢性痛中的作用
2、。方法選用60只成年雄性SD大鼠，將大鼠隨機(jī)分為對(duì)照組和實(shí)驗(yàn)組。實(shí)驗(yàn)組分別于術(shù)后1d、2d、3d、4d、5d處死;取雙側(cè)三叉神經(jīng)節(jié)、腦干三叉神經(jīng)脊束核、大腦皮層，應(yīng)用免疫組織化學(xué)檢測(cè)Integrin－αm表達(dá)情況，所獲數(shù)據(jù)經(jīng)方差分析和t檢驗(yàn)。結(jié)果對(duì)照組的雙側(cè)三叉神經(jīng)節(jié)、腦干三叉神經(jīng)脊束核、大腦皮層區(qū)可見(jiàn)極少量Integrin－αm表達(dá)，正畸治療加力后能誘導(dǎo)Integrin－αm在上述區(qū)域的表達(dá)，隨著加力時(shí)間的延長(zhǎng)(1～5d)，其表達(dá)水平逐漸增加，與對(duì)照組相比存在顯著差別(P＜0．01)。結(jié)論正畸治療加力后炎性相關(guān)因子Integ
3、rin－αm在三叉神經(jīng)節(jié)、腦干三叉神經(jīng)脊束核、大腦皮層表達(dá)增加，該作用提示Integrin－αm參與了錯(cuò)牙合矯正初期的炎癥反應(yīng)過(guò)程。關(guān)鍵詞:正畸學(xué);牙移動(dòng);Integrin－αm;疼痛;免疫組織化學(xué);SD大鼠中圖分類號(hào):R781．42文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):1008－4118(2010)04－0006－05AnStudyonExpressionofIntegrin－αmduringExperimentalToothMovementinRatsQiaoTing(QingdaoUniversityMedicalCollege，Qi
4、ngdao266021Shandong)Abstract:ObjectiveAnimalmodelsoforthodontictoothmovementwereestablishedinrats．ToobservetheexpressionofIntegrin－αmintrigeminalganglion(TG)，spinaltrigeminalnucleus(Vsp)andcerebralcortexofratsduringexperimentalorthodontictoothmovement，toinvestigatec
5、hronicpaininthenerveconductionpathwayofearlyorthodontictreatmentandthepossibleroleofIntegrin－αminchronicpain．Methods60SDratswererandomlydividedintocontrolandexperimentalgroups，nickel－titaniumspringswereplacedbetweentherightmaxillaryfirstmolarandmaxillarycentralincis
6、orsofrat．Theratswerekilledafter1d，2d，3d，4d，and5d．Rountinehistologicalstainingforviewchangesofhistomorphology，Integrin－αmexpressionwasexaminedwithimmunohistochemistrytechniqueintrigeminalgaglion(TG)，spinaltrigeminalnucleus(Vsp)andcerebralcortex．ResultsControlgroupsho
7、wsverylittleexpressionofIntegrin－αmintrigeminalgaglion(TG，spinaltrigeminalnucleus(Vsp)andcerebralcortex，orthodonticforcescaninduceIntegrin－αmexpressionintheseregions，withthetime(1～5d)，theexpres-sionofIntegrin－αmincreasedgradually，comparedwiththecontrolgrouptherewere
8、significantdifferences(P＜0．01)．ConclusionTheexpressionofIntegrin－αmtrigeminalganglion(TG)，spinaltrigeminalnucleus(Vsp)andcerebralcortexinc
