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1、心臟雜志(ChinHeartJ)2008,20(3)·301··基礎(chǔ)研究·α2亞麻酸對高糖導(dǎo)致的人臍靜脈內(nèi)皮細(xì)胞損傷的影響11211張薇,賈國良,王四旺,韓淑芳,布倫(第四軍醫(yī)大學(xué):1.西京醫(yī)院心血管內(nèi)科,陜西西安710032,2.藥物研究所,陜西西安710032)摘要:目的觀察α2亞麻酸(ALA)對高糖導(dǎo)致的人臍靜脈內(nèi)皮細(xì)胞(HUVECs)損傷的影響,探討ALA在糖尿病血管并發(fā)癥防治中的作用。方法采用體外培養(yǎng)的第2～4代HUVECs,分為6組,即正常對照組、高糖組(HG組)及HG+ALA(ALA濃度分別為10
2、、50、100和200μmol/L)組。各組細(xì)胞于不同培養(yǎng)環(huán)境中培養(yǎng)72h后收集標(biāo)本,用MTT比色法測定細(xì)胞活力,通過測定細(xì)胞上清液中一氧化氮(NO)和丙二醛(MDA)含量評估內(nèi)皮功能,末端脫氧核糖核酸酶介導(dǎo)的dUTP末端標(biāo)記法(TUNEL法)和流式細(xì)胞儀法檢測細(xì)胞凋亡情況。結(jié)果較低濃度(10、50、100μmol/L)的ALA組中,細(xì)胞存活率和合成NO濃度均顯著高于高糖組(P<0105),MDA濃度和細(xì)胞凋亡率顯著低于高糖組(P<0105);尤以50μmol/L時為著。當(dāng)ALA濃度增至200μmol/L時,細(xì)
3、胞存活率和合成NO濃度反而低于高糖組,MDA濃度和細(xì)胞凋亡率也較高糖組進(jìn)一步增加。結(jié)論ALA對高糖環(huán)境下培養(yǎng)的內(nèi)皮細(xì)胞有著雙重效應(yīng),既有保護(hù)作用也有細(xì)胞毒效應(yīng),與其濃度有關(guān)。關(guān)鍵詞:α2亞麻酸;糖尿病;人臍靜脈內(nèi)皮細(xì)胞;凋亡中圖分類號:R543.6;R587.1文獻(xiàn)標(biāo)識碼:A文章編號:100927236(2008)032301204Effectsofalpha2linolenicacidondamageofhumanumbilicalveinendo2thelialcellsinducedbyhighconce
4、ntrationglucose11211ZHANGWei,JIAGuo2liang,WANGSi2wang,HANShu2fang,BULun(1.DepartmentofCardiology,XijingHospital,2.InstituteofMateriaMedica,FourthMilitaryMedicalUniversity,Xi’an710032,Shaanxi,China)Abstract:AIMToinvestigatetheeffectsofalpha2linolenicacid(ALA)
5、ontheproliferationandfunc2tionofhumanumbilicalveinendothelialcells(HUVECs)inpresenceofhighconcentrationglucose.METHODSCulturedHUVECweredividedintosixgroups:controlgroup,highglucosegroup(HG),groupsofhighglucoseplusvariousconcentrationsofALA(10,50,100and200μmo
6、l/L).Cellsampleswerecollected72hourslaterandcellproliferationwasmeasuredbyMTTchromatometry.TheendothelialfunctionwasevaluatedbytheconcentrationofNOandMDAinculturemediumandapoptosiswasdeter2minedbypyridineiodidestainingonflowcytometryandtaggeddeoxynucleotidet
7、ransferase2mediateddUTPnickendlabeling(TUNEL).RESULTSPretreatmentwithlowconcentrationsofALA(10,50and100μmol/L)significantlyimprovedthecellviabilityandNOproductionanddecreasedMDAproductionandthenumberofapoptoticcells,withamaximaleffectat50μmol/L.Butincreasing
8、ALAto200μmol/Lproducedtheoppositeeffect.CONCLUSIONPretreatmentwithALAinducesabiphasicresponseincellgrowthandfunctionofHUVECsculturedinhighglucose2protectiveandcytotoxiniceffect,whichisrelatedtot
