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1、微衛(wèi)星DNA和AFLP標記在水稻分子標記連鎖圖上的分布1熊立仲1王石平1劉克德1戴先凱2M.A.SaghaiMaroof3胡錦國1張啟發(fā)1(華中農(nóng)業(yè)大學作物遺傳改良國家重點實驗室 武漢 430070)2(DepartmentofCropandSoilEnvironmentalScience,VirginiaPolytechnicInstituteandStateUniversity,VA24061,USA)3(DepartmentofvegetableCrops,UniversityofCalifornia,Davis,CA91616,USA)評論員:李宇歌摘要
2、以一個栽培稻(OryzasativaL.ssp.indica)和野生稻(O.rufipogonGriff)雜交的F2作圖群體以及由該群體構(gòu)建的RFLP標記連鎖圖,分析了微衛(wèi)星DNA和AFLP標記的多態(tài)性、遺傳行為及其在染色體上的分布。共定位了28個微衛(wèi)星DNA標記和172個AFLP標記。28個微衛(wèi)星DNA標記中有6個為華中農(nóng)業(yè)大學作物遺傳改良國家重點實驗室根據(jù)數(shù)據(jù)庫中序列而設(shè)計,其余22個來自美國Cornell大學已發(fā)表的結(jié)果。172個AFLP標記出自25對引物擴增得到的228個多態(tài)性帶的片段。這些標記分布于水稻的12條染色體。將此200個PCR標記與華中農(nóng)業(yè)大學
3、作物遺傳改良國家重點實驗室構(gòu)建的RFLP連鎖圖整合,得到一張含612個分子標記位點的遺傳連鎖圖。關(guān)鍵詞SSR,AFLP,RFLP,基因圖譜Abstract①SSR(simplesequencerepeat)andAFLP(amplifiedfragmentlengthpolymorphism)arePCRbasedmolecularmarkersdevelopedinrecentyears.②Inthisstudy,theauthorsanalyzedthepolymorphisms,inheritanceanddistributionofSSRandAFLPma
4、rkersusinganF2populationfromacrossbetweencultivar“AijiaoNante”(OryzasativaL.ssp.Indica)andanaccessionofthecommonwildrice(O.rufipogonGriff).③Atotalof200newmarkerswereobtainedincluding28SSRand172AFLPmarkers.④Sixofthe28SSRmarkersweredevelopedbyNationalKeyLaboratoryofCropGeneticImprovemen
5、t(NKLCGI)usingDNAsequencesfromGenBankandtheother22werefromdatapublishedpreviously.⑤The172AFLPmarkerswerefromatotal228polymorphicbandsamplifiedusing25selectedprimercombination.⑥Mappingofthe200newmarkersusingNKLCGI’SpreviouslydevelopedRFLPmapbasedonthesameF2populationresolvedthesemarker
6、stoall12ricechromosomes.⑦IntegrationoftheSSRandAFLPmarkersintotheRFLPmapresultedinahighdensitymolecularlinkagemapcontaining612polymorphicloci.標題:◆微衛(wèi)星DNA和AFLP標記在水稻分子標記連鎖圖上的分布◆DISTRIBUTIONOFSIMPLESEQUENCEREPEATANDAFLPMARKERSINMOLECULARLINKAGEMAPOFRICE改正:方案一:SSR和AFLP標記再水稻分子標記連鎖圖上的分布方案二:D
7、istributionofMicrosatalliteDNAandAFLPMarkersinMolecularMarkerLinkageMapofRice①不一一對應中文摘要第一句話是“以一個栽培稻(OryzasativaL.ssp.indica)和野生稻(O.rufipogonGriff)雜交的F2作圖群體以及由該群體構(gòu)建的RFLP標記連鎖圖,分析了微衛(wèi)星DNA和AFLP標記的多態(tài)性、遺傳行為及其在染色體上的分布”。英文摘要第一句是SSR(simplesequencerepeat)andAFLP(amplifiedfragmentlengthpolymorphi
8、sm)ar