資源描述：
《京尼平交聯(lián)脫細(xì)胞豬膀胱的體外生物學(xué)性能研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫(kù)。
1、京尼平交聯(lián)去脫細(xì)胞豬膀胱基質(zhì)生物材料的體外生物學(xué)性能研究范雪梅1,2王穎楠1陳衛(wèi)軍3徐惠成1［通訊作者］徐惠成，E-mail:xhuicheng@yahoo.com.cn?（400038重慶第三軍醫(yī)大學(xué)西南醫(yī)院婦產(chǎn)科1；610083成都成都軍區(qū)總醫(yī)院婦產(chǎn)科2；400038重慶第三軍醫(yī)大學(xué)解剖教研室，重慶市生物力學(xué)重點(diǎn)實(shí)驗(yàn)室3）［摘要］目的探討新型生物交聯(lián)劑京尼平對(duì)脫細(xì)胞豬膀胱性能的影響，為女性盆底功能重建提供理想的組織工程補(bǔ)片。方法采用表面活性劑+酶消化法對(duì)豬膀胱進(jìn)行脫細(xì)胞處理后，用0.625%京尼平溶液交聯(lián)脫細(xì)胞膀胱72小時(shí)。通
2、過(guò)HE和ET+VG染色及掃描電鏡進(jìn)行組織形態(tài)觀察，使用萬(wàn)能材料試驗(yàn)機(jī)檢測(cè)材料生物力學(xué)性能，并對(duì)交聯(lián)前后的脫細(xì)胞豬膀胱進(jìn)行厚度、吸水性、體外膠原酶降解性能力測(cè)定，以L929細(xì)胞為實(shí)驗(yàn)細(xì)胞，采用MTT法評(píng)估材料的細(xì)胞毒性。結(jié)果京尼平交聯(lián)脫細(xì)胞豬膀胱后材料呈深藍(lán)色，保持了天然組織構(gòu)架的完整，膠原纖維更加致密，吸水性提高，力學(xué)性能良好，脫細(xì)胞豬膀胱在第7天已完全降解，交聯(lián)后在第28天降解率2%。交聯(lián)前后材料的細(xì)胞毒性為0～1級(jí)。結(jié)論經(jīng)天然交聯(lián)劑京尼平交聯(lián)的脫細(xì)胞豬膀胱材料吸水性好，具有良好的生物力學(xué)性能，抗酶解能力強(qiáng)且無(wú)細(xì)胞毒性，是一種較
3、為理想的盆底重建材料。［關(guān)鍵詞］京尼平；脫細(xì)胞豬膀胱；交聯(lián)；力學(xué)性能；細(xì)胞毒性［中圖分類號(hào)］:R318.08；R318.16；R711.2CharacteristicsofAcellularPorcineBladderCrosslinkedwithGenipin（1DepartmentofObstetricsandGynecologyy,SouthwesternHospital,ThirdMilitaryMedicalUniversity,Chongqing400038；2DepartmentofObstetricsandGyne
4、cologyy，ChengduMilitaryGeneralHospital,Chengdu610083;DepartmentofAnatomy,CollegeofBasicMedicalSciences，ThirdMilitaryMedicalUniversity,Chongqing400038，China)［Abstract］ObjectiveToinvestigatethecharacteristicsandcytocompatibilityofacellularporcinebladderfixedwithgenipini
5、nvitro,andtoprovidetheideal16biomaterialsforthereconstructionofvefemalepelvicsurgery.MethodsThecCellularcomponentsoftheporcinebladderwereremovedbythedetergent-enzymaticmethod.andtTheacellularporcinebladderwaserecrosslinkedwith0.625%genipinfor72h,thenexaminedthemorphol
6、ogywithnaked-eyes,stainedwithhematoxylinandeosinstaining,elastin/vanGiesonstainingandscanningelectronmicroscope.Subsequently,thebiomechanicalstestswerealsoperformed.Propertiesofthecrosslinkedacellularmatrixwereevaluatedbytissuethicknessmeasurement,，waterabsorptionsoak
7、agecontentinandinvitroenzymaticdegradationtest.Thecytotoxicityofthegenipin-fixedmaterialswasevaluatedinvitrobyMTTassayusingamurineouse-derivedestablishedfibroblastcelllinecelllineofL-929fibroblastsastestcells.ResultsThegenipin-crosslinkedacellularporcinebladderwasdark
8、blue,withverywellpreservedcollagenfiber.Cosslinkedtissueshadstronghydrophilicityandgoodmechanicalproperties,thedegradationra
