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1、Cell.Mol.LifeSci.(2010)67:749767DOI10.1007/s00018-009-0192-2CellularandMolecularLifeSciencesREVIEWProgressinphagedisplay:evolutionofthetechniqueanditsapplicationsTomazˇBratkovicˇReceived:7September2009/Revised:18October2009/Accepted:23October2009/Publishedonline:15No
2、vember2009óBirkha¨userVerlag,Basel/Switzerland2009AbstractPhagedisplay,thepresentationof(poly)pep-Phagedisplayisatermdescribingdisplayofforeigntidesasfusionstocapsidproteinsonthesurfaceof(poly)peptidesonthesurfaceofphageparticle.Thisisbacterialviruses,celebratesits25
3、thbirthdayin2010.Theachievedbysplicingageneencodingsuchapeptideintoatechnique,coupledwithinvitroselection,enablesrapidgeneencodingacapsidstructuralprotein.Thecapsididenti?cationandoptimizationofproteinsbasedontheirproteinshouldideallyserveonlyasananchorforthestructur
4、alorfunctionalproperties.Inthelasttwodecades,displayedpeptideandnotinterferewithitsstructure;theithasadvancedtremendouslyandhasbecomewidelyideaistohaveapeptidetetheredtoareplicatingparticleacceptedbythescienti?ccommunity.Thisbynomeansviaapeptidelinker,yetretainingits
5、structure/functionasitexhaustivereviewaimstoinformthereaderofthekeywouldbefreeinsolution.UsingrecombinantDNAtech-modi?cationsinphagedisplay.Noveldisplayformats,nology,collectionsofbillionsofpeptides,proteinvariants,innovativelibrarydesignsandscreeningstrategiesaregen
6、efragment-orcDNA-encodedproteinspresentedondiscussed.Iwillalsobrie?yreviewsomerecentusesofthephage(so-calledphage-displayedlibraries)canbecon-technologytoillustrateitsincredibleversatility.structedandsurveyedforspeci?caf?nityoractivity(Fig.1).Severalexcellentbooksdea
7、lingwithdesign,KeywordsPhagedisplayMolecularevolutionconstruction,andapplicationsofphagedisplaylibrariesLibraryscreeningLibrarydesignSelectionstrategyhavebeenpublished[711].Relatedtechnologiesincluderibosomedisplay[12,13],mRNAdisplay[13],displayonthesurfaceofbact
8、erial[14]oreukaryoticcells[1517],Thetechnologyandoncapsidsofeukaryoticviruses[17,18].Thesehavebeenextensivelyreviewedelsewhereandar
