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1、_Archives.........ArchVirol(t995)140:571-579Virology?Springer-Verlagt995PrintedinAustriaSequenceanalysisofthenucleoproteingenesofthreeenterotropicstrainsofmurinecoronavirusBriefReportF.R.HombergerInstituteofLaboratoryAnimalScience,UniversityofZurich,Zurich,SwitzerlandAcceptedNovember1
2、5,1994Summary.Thenucleotidesequencesofthenucleoproteingenesofthreeenterotropicstrainsofthemurinecoronavirusmousehepatitisvirus(MHV-Y,MHV-RIandDVIM)weredeterminedandcomparedwithpreviouslyreportedsequencesofthreepolytropic(respiratory)strains(MHV-A59,MHV-JHMandMHV-S).Greaterthan92%homol
3、ogywasfoundamongthesixstrainsbypair-wisecomparisonatthenucleotidelevel.Thegenesencodedproteinsof451to455residuesandthededucedaminoacidsequencesweremorethan91~ohomologous.AuniquedeletionoftwelvenucleotideswasfoundatthecarboxyterminusofMHV-YandathreenucleotidedeletionwasfoundinMHV-RI,wh
4、ichcorrespondedtotheonepreviouslyreportedinMHV-A59andMHV-S.Twointernalopenreadingframeswerefoundwithinthecodingregionofthenucleoprotein,thesmalleronewasspecificfortheenterotropicstrains.ItcouldpotentiallyencodeatruncatedversionofthehypotheticalproteindescribedforMHV-A59andMHV-S.Sequen
5、cerelationshipoftheNgeneshowednocorrelationwithtissuetropismandnosequenceorevensingleaminoacidchangeuniquetoeithertropismgroupwasfound.ThisindicatesthatthenucleoproteinofMHVprobablyhasnopartinthedeterminationoftheprimarytissuetropismofanMHVstrain.Theroleofthepotentialinternalproteinwa
6、rrantsfurtherinvestigation.Mousehepatitisvirusisanimportantpathogenofthelaboratorymouseandthemostcommonvirusdetectedincontemporarymousecoloniesthroughouttheworld[17,21]'.Itsimplicationsonbiomedicalresearcharewelldocumented[-2,11].MHVisanRNAvirusbelongingtothecoronavirusgroup.Itishighl
7、ymutableandconsistsofanumberofantigenicaUydifferentstrainswhichvarymarkedlyinvirulenceandtissuetropism.Accordingtotheirtropismthestrains572F.R.Hombergercanbedividedintotwomaingroupswithdifferentdiseasepatterns[2].Onebiotype,therespiratoryorpolytropicstrains,primarilyinfectsthemucosaof
8、theup
