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1、AdvancePublicationTheJournalofVeterinaryMedicalScienceAcceptedDate:23July2019J-STAGEAdvancePublishedDate:23August2019?2019TheJapaneseSocietyofVeterinaryScienceAuthormanuscriptshavebeenpeerreviewedandacceptedforpublicationbuthavenotyetbeenedited.11Virology2Note34Felinecoro
2、navirusisolatesfromapartofBrazil:insightsintomolecularepidemiology5andphylogenyinferredfromthe7bgene67INSIGHTSINTOBRAZILIANFELINECORONAVIRUS89LucianaMYRRHA1?FernandaSILVA1?PedroVIDIGAL2?MaurícioRESENDE3?10GustavoBRESSAN1?JulianaFIETTO1?MarcusSANTOS4?LauraSILVA4?Viviane11A
3、SSAO4?AbelardoSILVAJUNIOR4?MárciaDEALMEIDA11213Address:FederalUniversityofVi?osa(UFV),PeterHenryRolfsAvenue,Vi?osa,14MinasGerais36570-900,Brazil15161LaboratoryofAnimalMolecularInfectology,InstituteofBiotechnologyAppliedto17Agriculture,FederalUniversityofVi?osa(UFV),Vi?osa
4、,MinasGerais36570-900,18Brazil192NucleusofAnalysisofBiomolecules,CenterofBiologicalSciences,Federal20UniversityofVi?osa(UFV),Vi?osa,MinasGerais36570-900,Brazil213DepartamentofMicrobiology,FederalUniversityofMinasGerais(UFMG),Belo22Horizonte,MinasGerais31275-035,Brazil234L
5、aboratoryofImmunobiologicalandAnimalVirology,DepartamentofVeterinary,24FederalUniversityofVi?osa(UFV),Vi?osa,MinasGerais36570-900,Brazil25226Correspondingauthor27AbelardoSilvaJúnior28DepartamentofVeterinary,FederalUniversityofVi?osa(UFV)29PeterHenryRolfsAvenue,Vi?osa,Mina
6、sGerais36570-900,Brazil30Phone+55313899147131E-mailabelardo.junior@ufv.br32333435363738394041424344454647484950351ABSTRACT52TheFelinecoronavirus(FCoV)canleadtoFelineinfectiousperitonitis(FIP),53whichtheprecisecauseisstillunknown.Thetheoryofinternalmutationsuggests54thatal
7、essvirulentbiotypeofFCoV(FECV)wouldleadtoanothermorepathogenic55biotype(FIPV)capableofcausingFIP.Inthiswork,the7bgenewasamplified56from51domesticcatplasmasamplesbysemi-nestedPCRandtestedthrough57phylogeneticandphylogeographicalapproaches.The7bgeneofBrazilianisolates58disp
8、layedhighconservation,astrongcorrelationbetweenthegeographicoriginof59theviralisolatesandtheirge
