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1、VIROLOGY169,217-221(1989)PhylogenyofAntigenicVariantsofAvianCoronavirusIBVJ.G.KuSTERS,*~t+H.G.M.NIESTERS,t2J.A.LENSTRA,*M.C.HORZINEK,tANDB.A.M.VANDERZEIJST*Departmentsof*BacteriologyandtVirology,InstituteofinfectiousDiseasesandimmunology,UniversityofUtrecht,P.0.Box80.165,3
2、508TDUtrecht,TheNetherlands,and*InstituteofMolecularBiologyandMedicalBiotechnology,UniversityofUtrecht,P.0.Box80.063,3508TBUtrecht,TheNetherlandsReceivedJuly14,1988;acceptedNovember7,1988ThesequencesofthepeplomericSlproteinoffourserologicallydistinctstrainsoftheinfectiousb
3、ronchitisvirus(IBV),anaviancoronavirus,havebeendetermined.TheSiproteinisthoughttocontaintheserotype-specificneutral-izationepitopesandtobethemaintargetofantigenicvariation.Analignmentwithsequencesofthreestrainspublishedpreviouslyshowedthatfromthe545aminoacidresiduesonly243
4、havebeenconserved.Clusteringofsubstitutionssuggeststhatmostserotypedeterminantsarelocatedwithinthefirst300aminoacidresiduesofSl.AphylogenetictreeoftheSlsequencesshowedveryvariableratesofdivergence.DifferencesintopologywithatreebasedonRNAse-Tlfingerprintdataindicatethatsome
5、oftheIBVstrainshavearisenbygeneticrecombination.o1wgAcadem;cPW.~,inc.Avianinfectiousbronchitis(16)isaworldwidedis-findingsthatallstronglyneutralizingmonoclonalanti-ease,whichiscausedbyacoronavirusandresultsinbodiesrecognizeSl,andthatimmunizationwithpuri-ahighlycontagiousre
6、spiratoryafflictionofyoungfiedSl,butnotwithviruslackingSl,inducesneutraliz-chickensorinadecreaseineggproduction(35).Ini-ingantibodies(5,7,29).tially,IBwaseffectivelycontrolledbyvaccinationwithToinvestigatetheserotypicvariationofIBVattheliveattenuatedIBvirus(IBV).However,th
7、isdidnotpre-molecularlevel,wedeterminedtheSlsequencesofventoutbreakscausedbyvariantviruses(10,13).fourIBVstrains(73,24):H120,anattenuatedvaccineRNaseTlfingerprintinganalysisshowedthatfieldstrainofserotypeA;D207,thereferencestrainofsero-strainsisolatedfromsuchoutbreaksarere
8、latedtovac-typeB;D1466,avaccinestrainofserotypeC;andcinestrains(24)suggestingthatthenewst
