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1、ArchVirol(2000)145:2135–2148EvidenceofgeneticdiversitygeneratedbyrecombinationamongaviancoronavirusIBVC.-W.LeeandM.W.JackwoodDepartmentofAvianMedicine,CollegeofVeterinaryMedicine,TheUniversityofGeorgia,Athens,Georgia,U.S.A.AcceptedMarch24,2000Summary.Previously,wedemons
2、tratedthattheDE072strainofIBVisarecom-binantwhichhasanIBVstrainD1466-likesequenceintheSgene.Herein,weanalyzedtheremaining3.8kb30endofthegenome,whichincludesGene3,Gene4,Gene5,Gene6,andthe30non-codingregionoftheDE072andD1466strains.Thosetwoviruseshadhighnucleotidesimilari
3、tyinGene4.However,theotherindividualgeneshadamuchdifferentlevelofsequencesimilaritywiththesamegeneoftheotherIBVstrains.Thegenomeof?veIBVstrains,ofwhichthecom-pletesequenceofthe30endofthegenomehasbeendetermined,weredividedatanintergenic(IG)consensussequence(CTGAACAAorCTT
4、AACAA)andcom-paredphylogenetically.PhylogenetictreesofdifferenttopologyindicatedthattheconsensusIGsequencesandthehighlyconservedsequencearoundthisregionsmayserveasrecombination`hotspots'.PhylogeneticanalysisofselectedregionsofthegenomeoftheDE072serotype?eldisolatesfurth
5、ersupportthoseresultsandindicatethatisolateswithinthesameserotypemayhavedifferentamountsofnucleotidesequencesimilaritywitheachotherinindividualgenesotherthantheSgene.PresumablythisoccursbecausetheconsensusIGsequenceservesasthetemplateswitchingsitefortheviralencodedpolym
6、erase.IntroductionInfectiousbronchitisviruscausesahighlycontagiousupper-respiratorydiseaseinchickens.Thediseaseischaracterizedbyincreasedocularandnasalsecre-tions,excessmucusinthetrachea,decreasedweightgainandfeedef?ciencyinbroilers,anddeclinesineggproductionandeggquali
7、tyinlayers.Althoughliveattenuatedvaccinesareavailable,IBVcontinuestobeasevereeconomicproblemincommercialchickensbecausemanydifferentserotypesofthevirusexistanddonotcrossprotect[3].2136C.-W.LeeandM.W.JackwoodInfectiousbronchitisvirus(IBV)isacoronavirusintheneworderNidovi
8、rales[4].MembersoftheNidoviralesorderhaveasinglestrandedpositivesenseRNAgenomeandproducea30nestedsetofsubgenom
