資源描述：
《冠狀病毒Coronavirusl論文-1995 Experimental Evidence of Recombination in Coronavirus Infectious Bronchitis Virus.pdf》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、VIROLOGY213,569–580(1995)ExperimentalEvidenceofRecombinationinCoronavirusInfectiousBronchitisVirus1SANNEKEA.KOTTIER,DAVIDCAVANAGH,andPAULBRITTONDivisionofMolecularBiology,InstituteforAnimalHealth,Compton,Newbury,Berkshire,RG207NN,UnitedKingdomReceivedAugust8,1995;acceptedSe
2、ptember6,1995Embryonatedeggswerecoinfectedwithtwostrainsofthecoronavirusavianinfectiousbronchitisvirus(IBV),IBV-BeaudetteandIBV-M41,toinvestigatewhetherrecombinationbetweenthetwostrainswouldoccur.VirionswereisolatedfromtheallantoicfluidofthecoinfectedeggsandputativehybridRN
3、Asweredetectedbypolymerasechainreaction(PCR),usingstrain-specificoligonucleotides.PCRproducts,oftheexpectedsizes,wereobtainedaspredictedfrompotentialrecombinationeventsbetweenthenucleoprotein(N)geneandthe3*-untranslatedregionofthetwoIBVgenomes.Sequenc-ingconfirmedthattheyco
4、rrespondedtohybridRNAs.VirusproducedasaresultofthemixedinfectionwastreatedwithanM41-specificneutralizingmonoclonalantibodyandpassagedinVerocells,inwhichIBV-Beaudette,butnotIBV-M41,replicated.HybridRNAwasstilldetectableafterthreeserialpassages.SincenoIBV-M41wasdetectablethis
5、confirmedthatinfectiousrecombinantgenomeshadbeenproducedintheembryonatedeggs.Thesefindingsnotonlysupportthecircumstantialevidence,fromsequencingstudiesofIBVfieldstrains,thatrecombinationoccursduringreplicationofIBVandcontributestothediversityofIBV,butalsoshowthatcoronavirus
6、RNArecombinationisnotlimitedtomousehepatitisvirus.q1995AcademicPress,Inc.INTRODUCTIONArecombinationmap,usingtemperature-sensitiveMHVmutants,revealedthefrequencyofrecombinationtobeInfectiousbronchitisvirus(IBV)isamemberoftheapproximately1%per1300nucleotides.AssumingthatCoron
7、aviridae(forreviewsseeLai,1990;Cavanaghetrecombinationoccursrandomly,thefrequencyofrecom-al.,1994)andcontainsasingle-strandedpositive-sensebinationintheentiregenomewasestimatedtobeRNAgenomeof28kb(Boursnelletal.,1987).Itisaaround25%(Baricetal.,1990).highlyinfectiousandcontag
8、iouspathogenofchickensAlthoughseveralsequencingstudiesonfieldisolateswhichreplicat
