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1、BlackwellPublishingLtdOxford,UKCMICellularMicrobiology1462-5814?2006TheAuthors;Journalcompilation?2006BlackwellPublishingLtd20068711471157OriginalArticleB.K.Doveetal.NucleolarperturbationinducedbycoronavirusinfectionCellularMicrobiology(2006)8(7),1147–1157doi:10.1111/j.1462-5822.200
2、6.00698.xFirstpublishedonline3March2006Changesinnucleolarmorphologyandproteinsduringinfectionwiththecoronavirusinfectiousbronchitisvirus111BrianK.Dove,Jae-HwanYou,MarkL.Reed,olarproteinstofacilitatevirusinfectionorusurphostcell12StevanR.Emmett,GavinBrooksandfunction(Hiscox,2002;2003
3、;RowlandandYoo,2003;1,3JulianA.Hiscox*Weidmanetal.,2003;Kimetal.,2004).Proteomicanal-1InstituteofMolecularandCellularBiology,Facultyofysisrevealedthatthenucleolusiscomposedofover400BiologicalSciences,UniversityofLeeds,Leeds,UK.proteinswhichcanbegroupedintodiscretefunctional2Schoolof
4、Pharmacy,UniversityofReading,Reading,UK.classesrelatedtotheactivityofthenucleolus(Andersen3AstburyCentreforStructuralMolecularBiology,etal.,2002;Leungetal.,2003).PredominantamongUniversityofLeeds,Leeds,UK.theseisnucleolin,whichcanaccountforapproximately10%oftheproteincontentwithinth
5、enucleolus.Nucleolinisamulti-domainandmultifunctionalproteinthathasbeenSummaryshowntobeinvolvedinribosomeassembly,rRNAmatu-Thenucleolusisadynamicsubnuclearstructureration,nucleo-cytoplasmictransportandcellproliferationinvolvedinribosomesubunitbiogenesis,cellcycle(Ginistyetal.,1998;S
6、rivastavaandPollard,1999).Nucle-controlandmediatingresponsestocellstress,olinishighlyphosphorylatedwiththepotentialtobindtoamongotherfunctions.WhilemanydifferentvirusesmultipleRNAtargetsandthismayre?ectitsvarietyoftargetproteinstothenucleolusandrecruitnucleolarfunctions(Ginistyetal.
7、,1999).Whilemammaliannucleo-proteinstofacilitatevirusreplication,theeffectoflinhasapredictedmolecularmassofapproximatelyinfectiononthenucleolusintermsofmorphology77kDa(dependingonthespecies),theapparentmolec-andproteincontentisunknown.Previouslywehaveularmassisbetween100and110kDa,an
8、dhasbeenshownthatth
