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《冠狀病毒Coronavirusl論文-1990 Sequence evidence for RNA recombination in field isolates of avian coronavirus infectious bronchitis virus.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、SequenceevidenceforRNArecombinationinfieldisolatesofaviancoronavirusinfectiousbronchitisvirusJ.G.Kusters*t,E.J.Jager*,H.G.M.Niesters~§andB.A.M.vanderZeijst~Underlaboratoryconditionscoronaviruseswereshowntohaveahighfrequencyofrecombination.InTheNetherlands,vaccinationagainst
2、infectiousbronchitisvirus(IB1I)isper.formedwithvaccinesthatcontainseverallife-attenuatedvirusstrains.Thesehighlyeffectivevaccinesmaycreateidealconditionsforrecombination,andcouldthereforebedangerousinthelongterm.Thispaperaddressesthequestionofthefrequencyofrecombinationofav
3、iancoronavirusIBVinthefield.Amethodwassoughttodetectandquantifyrecombinationfromsequencedata.NucleotidesequencesofeightIBVisolatesinaregionofthegenomesuspectedtocontainrecombination,werealignedandcompared.Phylogenetictreeswereconstructedfordifferentsectionsofthisregion.Diff
4、erencesintopologybetweenthesetreeswereobserved,suggestingthatinthreeoutofeightstrainsinvivoRNArecombinanthadoccurred.Keywords:RNArecombinant;coronavirus;infectiousbronchitisvirusIntroductionnewlydetermined.BothstrainsareDutchfieldisolatesandareincludedinvaccinescurrentlyuse
5、dinTheInfectiousbronchitisvirus(IBV)causesconsiderableNetherlands.ThealignmentofthesequencessuggestsdamageinthepoultryindustrybyinfectionsofthethattheDutchfieldstrainD20712,theBritishfieldstrainrespiratorytractandthereproductiveorgans1.IBVis6/8213andtheJapanesefieldstrainKB
6、8523t4resultfromtheprototypestrainoftheCoronaviridae,afamilyofRNArecombinationevents.positivestrandedRNAviruseswithagenomeofabout28kb.ThisgenomecontainstheinformationforthreestructuralproteinsandanumberofenzymesinvolvedinMaterialsandmethodsvirusreplication(Figure2).Although
7、vaccinesofferTheoriginoftheIBVstrainsaswellastheproceduresprotection,theyareoftenmadeineffectivebytheusedforthecloningandsequencingofthepeplomercontinuousemergenceofnewserotypes2"3.Serotype-geneshavebeenpublishedpreviously3'11'15.Sequencespecificprotectiveimmunityisthoughtt
8、obemediateddatawereprocessedusingtheprogramsofMicrogenicbyantibodiestoaproteinonth
